BEIJING, March 26 (Xinhua) According to the real-time statistics of Johns Hopkins University in the United States, as of 6: 30 a.m. on March 26, Beijing time, there were more than 460,000 confirmed cases and 21,000 cumulative deaths in COVID-19.

　　Among them, the epidemic situation in the United States, Italy, Spain and other countries is still grim; Spain’s Deputy Prime Minister, Britain’s Prince Charles and other national politicians have successively "recruited"; Britain, France, etc. responded to the epidemic through various laws and proposals; New Zealand, Philippines, Democratic Republic of Congo and many other countries declared a state of emergency.

Data map: UN Secretary-General Guterres. China News Service reporter Liao Panshe

　　United Nations: All mankind must fight back together.

　　On March 25th, local time, the United Nations launched the "Global Humanitarian Response Plan for COVID-19 Epidemic", which aims to help the most vulnerable countries in the world to fight the epidemic, protect the lives of millions of people and prevent the virus from spreading in other countries.

　　UN Secretary-General Guterres said that Covid-19 is threatening all mankind, so all mankind must fight back together. It is not enough for a single country to take countermeasures.

　　Guterres and the heads of various agencies called on member countries to commit themselves to preventing the spread of Covid-19 in fragile countries and give maximum support to the response plan, so as to control the spread of the epidemic worldwide.

　　In addition, Tedros Adhanom Ghebreyesus, Director-General of the World Health Organization, praised the Japanese decision to postpone the Tokyo Olympic Games as "difficult and wise" in his speech on 25th. At the same time, he called on countries that have taken "city closure" measures to make good use of this opportunity and take action to deal with the epidemic.

On March 24th, local time, people walked past the Javits Center in new york, USA. China News Service reporter Liao Panshe

　　The epidemic situation in New York State is serious, and the deployment of US troops abroad has been suspended for 60 days.

　　According to Johns Hopkins University data, as of 6: 30 a.m. on March 26th, Beijing time, there were 65,285 confirmed cases and 926 deaths in the United States. Among them, the epidemic situation in New York State is the most serious, with more than 30,000 confirmed cases.

　　Cuomo, the governor of New York State, said that the number of COVID-19 tests in New York State has accounted for 25% in the United States, but the peak of confirmed cases has not yet arrived.

　　In addition, Louisiana has become another state in the United States that has been listed as a "major disaster area" due to the epidemic. Affected by the epidemic, New York, Washington, California and Iowa have been listed as "major disaster areas".

　　On the 25th, the US Secretary of Defense issued a stop order to the US military, demanding that all US troops stationed overseas stop all travel and action plans. The order will be executed for up to 60 days to prevent the epidemic from spreading among US troops.

　　This measure is the most extensive preventive order of the US military so far, but the downsizing of the US military in Afghanistan will not be disturbed and will continue to maintain the withdrawal of personnel.

　　Italy: collective infection occurred in four nursing homes

　　According to the data of the Italian Ministry of Health on the 25th, the number of confirmed cases in Italy increased to 74,836, with 7,503 deaths.

　　In the past few days, collective infections have occurred in four nursing homes in Rome and surrounding areas. Up to now, more than 160 people have been infected and 3 people have died, and the number of infected people is still increasing. All four old-age care institutions were also isolated.

　　Earlier, the Italian cabinet passed a new epidemic prevention and control law to increase punishment for violations of prevention and control measures. According to the new law, Italy’s national emergency lasts until July 31st, during which the epidemic prevention and control law can be further adjusted.

　　In an interview with the media, the Assistant Director-General of WHO, Ranieri Gueira, said that Italy may see the peak of the epidemic this week.

　　Spain: Deputy Prime Minister tested positive.

　　By the 25th, there were 7,937 new confirmed cases in Spain, with a total of 47,610 cases and 3,434 deaths. Spain has become the fourth country in the world where the number of confirmed cases has exceeded 40,000, and the epidemic situation in Europe is second only to Italy.

　　The Spanish Prime Minister’s Office said on the 25th that the Covid-19 test conducted by Spanish Deputy Prime Minister Carmen Calvo on the 24th was positive. At present, Calvo is receiving isolation treatment in a clinic in Madrid, and his recovery is good.

　　In addition, several officials of the Spanish government have been infected. The wife, mother and father-in-law of Spanish Prime Minister Sanchez were also diagnosed.

　　British Parliament Passes Epidemic Emergency Bill

　　On the 25th local time, the British Ministry of Health and Social Security released the latest data. There were 1,452 new confirmed cases and 43 new deaths in the country, with a total of 9,529 confirmed cases. The cumulative total number of deaths is 465.

　　The emergency bill to deal with the COVID-19 epidemic was passed in the upper house of the British Parliament on the same day, and it only needs to be passed by the royal family to become law. The bill aims to give the government more power to deal with the spread of the epidemic.

　　In addition, Buckingham Palace said on the 25th that Prince Charles Covid-19’s test results were positive, but his symptoms were mild, and he was in good health and was in isolation at home. Queen Elizabeth II is currently in good health.

　　Germany: Merkel’s second test was negative

　　As of 0: 00 local time on the 25th, there were 4,118 newly confirmed cases in Germany, with a total of 31,554 cases and 149 deaths.

Data map: German Chancellor Angela Merkel. China News Service reporter Peng Dawei photo

　　German Chancellor Angela Merkel’s second Covid-19 test was negative, and a third test will be conducted early next week, a German government spokesman announced on the 25th. Previously, she had contact with a confirmed patient and began to be isolated at home on the 22nd.

　　France passed 25 emergency decrees, and Switzerland was diagnosed with more than 10,000 cases.

　　On 25th, French President Macron visited the hardest-hit areas in France and announced the launch of anti-epidemic military operations. The French epidemic continued to deteriorate that day, with the cumulative number of confirmed cases rising to 25,233 and the number of deaths rising to 1,331.

Under strict control measures, there are almost no pedestrians and vehicles on Rivoli Avenue in the center of Paris. China News Service reporter Yang Li photo

　　French Prime Minister Philippe announced on the same day that the meeting passed 25 administrative decrees to deal with the impact of the epidemic on all aspects of economy and society.

　　On the other hand, according to the data of Hopkins University, as of 6: 02 Beijing time on the 26th, Switzerland has diagnosed 10,897 cases, making it the fifth country in Europe with more than 10,000 confirmed cases.

　　Russia: The whole people will have a paid holiday for one week from next week.

　　On the 25th local time, Russian President Vladimir Putin announced in his speech on the epidemic that Russia will have a paid holiday for one week next week, and the long holiday will last from March 28th to April 5th.

　　He said: "Of course, all living security institutions, including medical institutions, pharmacies, shops, banks, financial settlement institutions, transportation and power organs at all levels will continue to operate."

　　Affected by the epidemic, the referendum on Russian constitutional amendments will also be postponed.

　　Russian Deputy Prime Minister Gorikova said on the 25th that the cumulative number of confirmed cases in Russia has increased to 658, and 29 cases have been cured.

　　Many countries declared a state of emergency.

　　New Zealand announced on the 25th local time that the country entered a state of emergency that day. The state of emergency applies to the whole of New Zealand, including chatham islands, Stewart Island and other inhabited islands.

　　On the same day, the mayor of Ottawa, Canada, Jim Watson announced that Ottawa had entered a state of emergency. Prior to this, Toronto, Vancouver, Calgary and other big cities have successively declared a state of emergency.

　　In response to the epidemic, the Philippines, Ukraine, Democratic Republic of the Congo, Ganel, C? te d ‘Ivoire, Sierra Leone and other countries have declared a state of emergency.

Every reporter | Zhang Rui every edited | Chen Xing　　　　

On June 21st, the press conference of the 2023 Global Digital Economy Conference was held in Beijing.

National business daily reporter learned that the 2023 Global Digital Economy Conference will be held at the Beijing National Convention Center from July 4th to 7th. With the theme of "data-driven development and intelligence leading the future", the conference provided a global platform for the exchange and cooperation of new theories, rules, technologies, industries and systems of the digital economy.

Introduction: The main forum of the conference will invite domestic and foreign experts, academicians and business representatives to deliver speeches, release the White Paper on Global Digital Economy, hold a summit dialogue on international cooperation in digital economy, and create a global weather vane for policy formulation, technological frontier, industrial trends and application paradigms in the digital economy.

Press conference scene Image source: Every reporter Zhang Rui photo

Introduce that the opening ceremony and main forum of this conference will further innovate the mode of holding the conference:

The first is to further improve the degree of internationalization and expand the scope of international cooperation and exchanges. Combined with the overall strengthening of international exchanges and cooperation in the post-epidemic period, it is planned to expand the invitation of international representatives to participate in the conference, set up the guest country of the conference for the first time, and issue the "Global Digital Economy Partner City Cooperation Initiative" to promote the joint construction of the "Digital Silk Road" and bilateral digital governance cooperation, so as to make the conference a "booster" for promoting digital open cooperation between cities around the world and under the international multilateral framework.

The second is to further optimize the degree of quality products, open up innovation and deepen cooperation. On the basis of previous exhibitions, the boutique theme exhibition will expand its exhibition area to 10,000 square meters, and set up digital infrastructure, data elements, industry digitalization, digital industrialization and digital security exhibition areas, highlighting professionalism, foresight, platform and experience, comprehensively enhancing its specifications and influence, and building a top-level international platform for digital economy frontier collision exchange and results release.

The third is to further demonstrate the charm of digital technology and start a number of "first time" attempts. For the first time, the "Digital Night" city pulse lighting ceremony was set up, and the video of the "Digital Night" city pulse lighting ceremony was broadcast live, interspersed with offline real scenes, and shared between reality and reality. At Shougang Park, Tongzhou Canal, Olympic Tower and Qianmen Dashilan, you can watch the AR show scene with a strong sense of science and technology.

Play the first China Digital Concert on the whole network at 21: 00, open a digital music feast, gather the top domestic virtual human technical team, top domestic virtual idols, virtual hosts, artists and other top resources, stage a cross-dimensional digital music festival, and show the world the Beijing business card and the vitality of an open, cooperative and win-win international city.

"It is worth mentioning that for the first time, the conference fully used digital badges to achieve paperless participation and create a new experience of digital participation." Say.

The fourth is to further highlight the degree of specialization and release major achievements. The White Paper on Global Digital Economy in 2023 will be released to promote the cooperation and exchange of global digital economy. Create a global vane of policy formulation, technological frontier, industrial trends and application paradigm in the digital economy.

At the press conference, he said that in recent years, under the strong leadership of the CPC Central Committee and the vigorous promotion of various departments and regions, China’s digital economy has achieved positive results.

The first is that the comprehensive strength of digital infrastructure has reached a new level. China has built the world’s largest and technologically advanced network infrastructure. By the end of April this year, more than 2.73 million 5G base stations have been built, with 5G networks covering all prefecture-level cities, more than 17.93 million ports with Gigabit network services, 634 million mobile phone users, 100 million Gigabit optical network users and 1.984 billion mobile Internet of Things users. 5G applications have covered 52 national economic categories, and the foundation of the Internet of Everything is stable.

Second, the integration of the digital economy and the real economy has become increasingly solid. The penetration rate of numerical control and digital R&D design tools for key processes in key industrial enterprises reached 59.4% and 77.6% respectively. The industrial Internet was fully integrated into 45 categories of national economy, with an industrial scale of over 1.2 trillion yuan. The "5G+ industrial Internet" has landed in thousands of industries and extended to the core links of production.

Third, the core digital industry is growing. In 2022, China’s electronic information manufacturing industry achieved operating income of 15.4 trillion yuan, software business income of 10.8 trillion yuan, and big data industry income of 1.57 trillion yuan, which laid a solid industrial and technical foundation for the integration and development of informationization and industrialization.

The incubation and application promotion of high-quality open source communities at home and abroad are accelerating, which has injected vitality into technological innovation and economic and social development.

The fourth is to speed up the flow of data elements. Thoroughly implement the "Twenty Data Articles", speed up the construction of a data-based system, and study and formulate national standards in the fields of data property rights, circulation transactions, asset evaluation, and security governance. Continue to implement the National Standard for Data Management Capability (DCMM). By the end of March 2023, a total of 1,469 enterprises had completed the standard implementation, covering 10 major fields such as electric power and communication.

Promote the high-level construction of data exchanges in Beijing, Shanghai and other places, with the rapid rise of emerging entities such as data labeling, data intermediary and data governance, and gradually form market operation systems such as data asset evaluation, registration and settlement, and transaction matching, and further release the potential of data elements.

Finally, the international cooperation in the digital economy has gone deep into reality. Actively carry out pragmatic cooperation with ASEAN, the African Union and countries along the Belt and Road in the field of digital economy, participate in the formulation of digital rules by multilateral mechanisms such as the United Nations, G20 and BRICS, and advocate the launching of a series of international cooperation initiatives and agreements, such as the G20 Digital Economy Development and Cooperation Initiative, the Joint Action Initiative to Build a Community of Cyberspace Destiny and the Global Data Security Initiative, which have significantly enhanced the international influence of China’s digital economy.

Cover image source: Photo courtesy of the organizer

Team Xiang Ye of Medical College made a breakthrough in the research of African swine fever virus.

Li Pilong’s research group of School of Life and Li Haitao’s research group of Medical College cooperated to report the new mechanism of histone modification regulating chromatin compartmentalization by promoting phase separation.

The Engineering Laboratory of Medical College reported the key factors regulating the spread of dengue virus and put forward a new anti-dengue blocking strategy.

The research group of Li Yue, Department of Electronics, issued a report on the realization of near-zero exponential concept devices by dielectric integrated optical doping.

Fang Xianyang’s research group of School of Life has made new progress in the solution structure study of long-chain non-coding subgenomic RNA of flavivirus.

Zhu Junming and Xue Lan of the School of Public Administration jointly published a document to reveal the impact of China’s carbon emission trading policy on low-carbon innovation.

Zhong Yi’s research group of School of Life reveals the downstream molecular mechanism of active forgetting of different memory components.

Xiao Bailong from the School of Pharmacy and Li Xueming from the School of Life jointly published a paper in Nature.

Reveal the delicate molecular machines structure and mechanism that mediates human tactile perception.

Team Xiang Ye of Tsinghua Medical College has made a breakthrough in the research of African swine fever virus.

On September 17th, a team led by Xiang Ye, an associate professor hired by Tsinghua University Medical Dean and a researcher at Beijing Advanced Innovation Center for Structural Biology, published an academic paper entitled "Structure of the African Swine fever virus major capsid protein p72" online in Cell Research. In this study, the high-resolution structure of African classical swine fever virus capsid protein p72 was analyzed for the first time by the method of three-dimensional reconstruction of single particle by cryoelectron microscope, and the possible assembly mechanism of African classical swine fever virus capsid was revealed, which laid a solid foundation for the development of African classical swine fever virus subunit vaccine.

African swine fever virus originated in Africa, and the mortality rate of domestic pig infection is nearly 100%, which is a great threat to domestic pig breeding industry and even people’s livelihood and economy. African swine fever virus has spread rapidly from Africa to Europe, South America and some parts of Asia in the past few decades. In August 2018, African swine fever virus infection was found in northeast China, and soon the virus spread to most parts of the country, causing economic losses of more than 100 billion yuan. At present, it is urgent to develop effective vaccines and epidemic prevention measures to control and prevent African swine fever virus. In response to the urgent needs of the country, Tsinghua University Beijing Advanced Innovation Center for Structural Biology and Beijing Frontier Research Center for Biological Structure took the lead, and more than a dozen research groups from Peking University, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Shanghai Pasteur Institute and other units began to tackle key problems of African swine fever virus in April this year.

▲ Structure of African classical swine fever virus: a. Simulate the capsid structure of African classical swine fever virus produced by virus assembly according to the capsid protein p72 structure of African classical swine fever virus; B freeze electron microscopic structure of African classical swine fever virus capsid protein p72 trimer.

African swine fever virus is a double-stranded DNA virus with complex structure. Its genome is protected by two capsids and two outer membranes. The capsids are mainly composed of virus coding protein p72. According to previous experimental data, p72 accounts for about 33% of the total weight of virus particles, and p72 is the main antigen that can be detected in the blood of infected pigs. At the initial stage of studying p72 protein, Xiang Ye’s research group found that p72 protein with correct folding conformation could not be obtained by recombinant expression of p72 protein alone, and only when p72 was co-expressed with another helper protein B602L encoded by African swine fever, could the p72 protein with correct folding conformation be obtained. On this premise, the research team determined the high-resolution 3-D structure of p72 protein of 2.67 angstrom (above) by using the method of single particle 3-D reconstruction with cryoelectron microscope. The results showed that three p72 molecules formed a stable trimer form. Each p72 contains two pancake-like domains connected in series, and each domain consists of eight β-folded sheets, which is very common in the capsid protein structure of icosahedral virus. The six cake-like structures of trimer p72 together form a pseudo-hexamer whose bottom is anchored on the inner membrane of the virus. The inserted fragments between trimer p72 folded sheets together form a propeller-like top structure that extends out of the virus, which is most likely the receptor binding area on the cell surface.

There have been many attempts to study subunit vaccines based on p72 protein of African classical swine fever virus, but no satisfactory results have been obtained. The failure of previous studies is probably due to the failure to use the correctly folded p72 protein. Obtaining the correctly folded p72 protein and determining its high-resolution structure are helpful to develop drugs targeting virus capsid assembly and subunit vaccines. At present, the research and development and evaluation of p72 subunit vaccine are being closely carried out by Tsinghua University and Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences.

Associate Professor Xiang Ye is the correspondent of this article. Liu Qi, a 2014 Ph.D. student from Tsinghua University Life Union Center, is the first author of this paper. Ma Bingting, a 2015 Ph.D. student from Xiangye Laboratory, Associate Professor Tan Xu from Tsinghua University College of Pharmacy and their laboratory members Qian Nianchao and Zhang Fan, and Professor Lei Jianlin, director of the cryo-electron microscope platform of Tsinghua University Life College, participated in this work.

Paper link:

https://www.nature.com/articles/s41422-019-0232-x

Li Pilong’s research group of Tsinghua Life College cooperated with Li Haitao’s research group of Medical College to report the new mechanism of histone modification regulating chromatin compartmentalization by promoting phase separation.

On September 16th, Li Pilong’s research group of Tsinghua University Life College cooperated with Li Haitao’s research group of Tsinghua University Medical College. An academic article entitled "Histone modifications regulate chromatin compartmentalization by contributing to a phase separation mechanism" was published in Molecular Cell.

Eukaryotic cells use many membrane-bound organelles to distinguish biochemical reactions in order to better regulate space and time. For example, their genomes are enclosed in a membrane-bound organelle, namely the nucleus, in order to better control the genetic information flow. In the nucleus, the genome is further regulated to form many compartments or regions, which play an important role in regulating gene expression. Judging from some of their characteristics, these dynamic solid compartments are similar to membraneless organelles. Many protein that play a role in chromatin compartmentalization have the inherent ability of liquid-liquid separation, such as human heterochromatin protein HP1α and Drosophila heterochromatin protein HP1a. Based on these observations, it has been suggested that chromatin compartmentalization is formed by liquid-liquid separation. However, the previous studies can’t explain why the other two homologous proteins, HP1β and HP1γ, of human heterochromatin protein HP1α, which can’t be separated by themselves or from DNA, perform similar functions, and whether the formation of constitutive heterochromatin is regulated by the liquid-liquid separation mechanism formed by multivalent interaction between multivalent HP1 protein complex and multivalent histone H3K9 trimethyl (H3K9me3).

▲ Liquid-liquid separation mediated by multivalent interaction between H3K9me3 on nucleosome string and CD of HP1 complex promotes the formation of heterochromatin compartments.

In this work, Li Pilong and Li Haitao’s research group cooperated to prove that in vitro, the modification of histone H3K9 trimethyl (H3K9me3) and the multivalent interaction of recognizing its modified HP1 chromatin domain Chromodomain(CD) can lead to the formation of heterochromatin. The authors used two polyvalent HP1-CD complexes, the key chromatin fragment (NE) extracted from human nucleus and the nucleosome string (NA) modified by H3K9me3 in vitro as experimental systems. Several evidences show that the formation of these droplets is mediated by liquid-liquid separation. The resulting droplets are reminiscent of the dynamic behavior of heterochromatin and its response to biochemical regulation and mutant phenotype. The author concludes that the liquid-liquid separation driven by multivalent H3K9me3/CD interaction is the key force for the formation of heterochromatin in cells. To sum up, this study proposed a general mechanism of histone labeling regulating chromosome compartmentalization by promoting liquid-liquid separation.

Li Pilong and Li Haitao are co-authors of this article. Wang Liang, a 2015 Ph.D. student from Tsinghua University Life College, Gao Yifei, a 2016 Ph.D. student from Medical College, and Zheng Xiangdong, a postdoctoral fellow from Medical College, are the co-first authors of this article.

Paper link:

https://www.cell.com/molecular-cell/fulltext/S1097-2765(19)30658-6

Cheng Gong Laboratory of Tsinghua Medical College reported the key factors regulating the spread of dengue virus and proposed a new anti-dengue blocking strategy.

On September 16th, Professor Cheng Gong from Tsinghua University Medical College published an academic paper entitled "Host serum iron modulations of dengue virus acquisition by mosquitoes" (doi: 10.1038/S41564-019-0555-) in Nature Microbiology. This study found for the first time that the iron content in human serum is the key factor to regulate the transmission of dengue virus by mosquitoes, and put forward an anti-dengue transmission blocking strategy based on iron supplementation.

Dengue fever is one of the most widespread viral infectious diseases in the world. Dengue virus is carried by mosquitoes and spread to people. At present, dengue fever has been prevalent in more than 100 countries and regions around the world. According to the estimation of the World Health Organization, about 2.5 billion people in the world live in areas threatened by dengue infection, 390 million people are infected or repeatedly infected with dengue virus every year, and 500-1 million people are hospitalized. Dengue fever has become the largest insect-borne viral infectious disease in the world. In recent 20 years, the epidemic trend of dengue fever in the world has intensified, which has become a worldwide public health problem. Aedes aegypti and Aedes albopictus are the primary vectors of dengue virus transmission in nature. This kind of mosquitoes are widely distributed in tropical and subtropical regions, but the epidemic trend of dengue fever is not completely consistent with the distribution of mosquitoes, and there are obvious population and regional differences in dengue fever. Identifying the key factors affecting the spread of dengue virus is a key scientific problem to be answered in this field.

Mosquitoes can acquire viruses from infected hosts by sucking blood. Within a few days after mosquitoes suck blood, the infected person’s blood is quickly digested. At the same time, the virus in the blood infects the intestinal epithelial cells of mosquitoes and spreads into the body cavity of mosquitoes, so that mosquitoes have the ability to spread viruses. The above two processes are highly coincident in time, so researchers speculate that host blood factors or blood metabolites can regulate the process of virus infection in mosquitoes, and ultimately determine the vector efficiency of mosquitoes carrying and spreading viruses. Through a series of screening, it was found for the first time that the iron ion content in the host serum was highly negatively correlated with the ability of mosquitoes to acquire virus by blood-sucking, and it was proved that the iron ion concentration in the serum was the key negative regulatory factor to inhibit mosquitoes from acquiring virus infection. The results of further research show that iron ions in serum can be directly utilized by the iron metabolism system of intestinal cells after entering the intestinal tract of mosquitoes, which inhibits the expression of antioxidant enzyme genes in cells, leading to a significant increase in the level of reactive oxygen species (ROS) in the intestinal tract of mosquitoes, and finally inhibits the infection and amplification of viruses in mosquitoes (Figure 1).

▲ Figure 1. Host serum iron ions can increase the level of reactive oxygen species in the intestinal tract of mosquitoes and inhibit the infection of dengue virus in mosquitoes by inhibiting the expression of antioxidant enzyme genes in intestinal cells.

Iron deficiency is a nutritional problem widely existing in nature. According to the World Health Organization, iron deficiency is widely distributed in Africa, South America, Southeast Asia and other regions. By comparison, it can be found that the distribution area of iron deficiency is highly coincident with the epidemic area of dengue fever, so the researchers speculate that the iron deficiency state of the population is closely related to the epidemic of dengue virus. In the subsequent study, it was found that the low iron state of the host can promote mosquitoes to infect the virus during blood feeding, and iron supplementation to the low iron host can block the spread of the virus. It shows that universal iron supplementation is an effective means to cut off the spread of dengue virus in nature, which will provide a new prevention and control idea and strategy for dengue virus prevention and control.

▲ Figure 2. Iron supplementation to the host can block mosquitoes from carrying and spreading dengue virus.

Professor Cheng Gong of Tsinghua University Medical College is the correspondent of this paper, and Zhu Yibin, a postdoctoral fellow of Tsinghua University Medical College, is the first author. Professor Wang Penghua from the Medical College of the University of Connecticut, Professor Liu Qiyong from the Institute of Infectious Diseases of the Chinese Center for Disease Control and Prevention, Professor Bian Zhongqi from the Department of Infectious Diseases of the 920th Hospital of China People’s Liberation Army Joint Logistics Support Force, Professor Itsanun Wiwatanaratanabutr from Thailand Xianhuang Institute of Technology, and Wu Tianshi, deputy chief physician of Tsinghua University School Hospital, are the collaborators of this paper.

Paper link:

https://www.nature.com/articles/s41564-019-0555-x

The research group of Li Yue, Department of Electronics, Tsinghua, published a report in Nature Communication on the realization of near-zero exponential concept devices through dielectric integrated optical doping.

On September 11th, the research group of Associate Professor Li Yue of Tsinghua University Department of Electronic Engineering published a research paper entitled "Substance-integrated photonic doping for near-zero-index devices" in Nature Communications. It is the first time to propose an optical doping method that can be integrated with dielectric to realize functional devices with near-zero exponential electromagnetic characteristics, which provides a new design method for new chip integrated circuits and has potential application value in biosensor, integrated circuits, functional materials and other fields.

▲ Conceptual diagram of dielectric integrated optical doping: (a) linear structure, (b) bending structure.

Near-zero-index device is a kind of functional device based on near-zero index medium. Compared with traditional electromagnetic devices, it has the characteristics of deformation, local adjustment and field enhancement. Electromagnetic wave in near-zero exponential medium presents a special wave effect of spatial static distribution, including near-zero refractive index and group velocity, as well as infinite wavelength and phase velocity. In 2017, Professor Nader Engheta and Associate Professor Li Yue of the Department of Electrical and Systems Engineering of the University of Pennsylvania jointly published an article in the journal Science, proposing the concept of optical doping based on near-zero exponential media for the first time, transplanting doping technology from micro-scale to macro-scale, and regulating the magnetic permeability of equivalent media through doping in non-ordered media, thus realizing multi-modal control such as paramagnetic, diamagnetic and ideal magnet. It is a kind of aperiodic structure.

▲ Experimental observation of optical doping characteristics of dielectric integration: (a) test platform, (b) near-zero reflection, zero phase and high group delay.

Based on the engineering application of the new theory, this paper puts forward an optical doping method based on dielectric integration. Combining with typical integrated circuit technology, the regulation of optical doping on the magnetic characteristics of equivalent media is expressed as the regulation of the electromagnetic response of chip integrated devices, and a variety of near-zero exponential functional devices are realized. For example, a high-sensitivity biosensor with the help of local field enhancement effect in doped media, an efficient photoacoustic modulator with weak mechanical vibration, and an "electric fiber" (analogous to optical fiber) that can be bent and deformed at will and its working frequency remains unchanged. In addition, Li Yue team verified the electromagnetic characteristics of the concept of dielectric integrated optical doping through experiments, and observed zero phase shift, high time delay, position independence of doped media and device shape independence, which were consistent with the analytical theoretical prediction and full-wave simulation results.

The theoretical and experimental work of this thesis was completed in Tsinghua University, and the Department of Electronic Engineering of Tsinghua University was the first unit of this thesis. Li Yue and Nader Enkhtar are the corresponding authors of this article, and Zhou Ziheng, a doctoral student in the Department of Electronic Engineering, is the first author. Other authors include Li Hao and Sun Wangyu, Ph.D. students from Tsinghua University Department of Electronic Engineering, and Dr. Inigo Liberal from Navarre Public University.

Paper link:

https://www.nature.com/articles/s41467-019-12083-y

Fang Xianyang’s research group of Tsinghua Institute of Life has made new progress in the study of solution structure of long-chain non-coding subgenomic RNA of flavivirus.

On September 10th, Fang Xianyang’s research group of the School of Life cooperated with Qin Chengfeng’s research group of the Institute of Microbiology and Epidemiology of the Academy of Military Medicine. A research paper entitled "Long non-coding subgenomic RNA of flavivirus has extended 3D structures and is flexible in solution" was published in EMBO Reports. In this paper, the solution structure of long-chain non-coding subgenomic RNA from flavivirus type 2 dengue virus, Zika virus and West Nile virus was reported in detail for the first time.

▲ Flow chart of studying the structure of flavivirus sfRNA solution by SAXS and computational simulation.

Long non-coding RNA (lncRNA) is a non-coding RNA with a length of more than 200 nucleotides, which plays a variety of important functions in organisms and is a hot spot in life science research. Like its host, almost all viruses can encode long-chain noncoding RNA. Many mosquito-borne flavivirus, such as dengue virus, Zika virus and West Nile virus, are pathogens that pose a great threat to human health. A large number of long-chain non-coding subgenomic RNA(sfRNA) will accumulate in the infected host cells, which play an important role in the pathogenicity of the virus and the immune escape from the host.

It is of great significance to study the structure-function of long-chain noncoding RNA, but at present, there is no effective means to study its three-dimensional spatial structure, so the three-dimensional spatial structure information of long-chain noncoding RNA is still very little. The length of long-chain non-coding subgenomic RNA from mosquito-borne flavivirus is between 400 and 500 nucleotides, so it is very difficult to study its structure by using traditional structural biology methods, such as X-ray crystallography, nuclear magnetic resonance or cryoelectron microscopy. Fang Xianyang’s research team and their collaborators, combined with small-angle X-ray scattering technology and computational simulation, studied the solution structure and flexibility of long-chain non-coding subgenomic RNA from dengue virus type 2, Zika virus and West Nile virus by step-by-step solution. It is found that the long non-coding subgenomic RNA of flavivirus has multiple subdomains, which have an extended three-dimensional spatial structure in solution, modular structural characteristics similar to multi-domain proteins and great flexibility.

It is particularly interesting that the three-dimensional spatial shapes and structures of the dumbbell-shaped domains in series of sfRNA of Dengue virus, Zika virus and West Nile virus based on small-angle X-ray scattering data are significantly different, which is presumed to be caused by the topological differences of their pseudo-knots in series. This inference is confirmed by further computational simulation, in vitro biochemistry and in vivo cell experiments. This study is an active exploration of the three-dimensional structure of long-chain noncoding RNA. The research results of this paper not only provide important structural information for understanding the physiological function of sfRNA, but also show that the integrated structural biology method based on small-angle X-ray scattering and computational simulation is a feasible method for studying the three-dimensional structure of other long-chain noncoding RNA solutions.

Fang Xianyang, researcher of Tsinghua University Life College, and Qin Chengfeng, researcher of Institute of Microbial Epidemiology, Institute of Military Medicine, Academy of Military Sciences, are co-authors of this article. Zhang Yupeng, a 2013 direct doctoral student of Tsinghua University Life College, Zhang Yikan, a 2016 general doctoral student, Dr. Liu Zhongyu, an associate professor of Institute of Microbial Epidemiology, and Cheng Mengli, a master student of Institute of Microbial Epidemiology, are co-authors of this article.

Paper link:

https://doi.org/10.15252/embr.201847016

Zhu Junming and Xue Lan of Tsinghua University of Public Administration jointly published a document to reveal the impact of China’s carbon emission trading policy on low-carbon innovation.

On September 9th, Associate Professor Zhu Junming and Professor Xue Lan from School of Public Administration of Tsinghua University published a research paper entitled "Low-carbon innovation induced by emissions trading in China" online in the international academic journal Nature Communications, and evaluated the effect of the pilot policy of carbon emissions trading in China on promoting enterprise innovation and its mechanism.

Carbon emission trading is a widely concerned market-based carbon emission reduction policy, which has been adopted by many countries and regions in the world and can play an important role in mitigating climate change. At present, the empirical knowledge about the effect of carbon emissions trading policy mainly comes from the study of EU carbon market. However, people still have little understanding of the scope of this market-oriented policy, the influence of interaction with other policies and the changes in the mechanism brought about by policy design, which limits the further promotion and greater role of the policy.

In order to solve this problem, Associate Professor Zhu Junming and Professor Xue Lan from School of Public Administration of Tsinghua University pay close attention to the pilot policy of carbon emission trading in China since 2013, and use enterprise-level data and patent application information to empirically test the influence and potential mechanism of the policy on low-carbon innovation of enterprises by using the methods of matching and double difference. Low-carbon innovation is helpful to get rid of the path dependence on carbon-intensive economy and energy infrastructure, and alleviate the negative impact caused by the uncertain trend of carbon emission reduction, which is very important to achieve stable emission reduction.

▲ Patent application trends of carbon trading enterprises and non-carbon trading enterprises.

It is found that, on the basis of the impact of policies such as energy conservation and emission reduction and low-carbon pilot projects, carbon emission trading has promoted trading enterprises to increase 1.75 low-carbon patent applications within two years, which is close to the policy effect of two low-carbon patent applications in the EU carbon market within five years, and at the same time, it has increased the applications of other types of patents, especially other green technology patents. The influence scope of the policy goes beyond the carbon emission trading enterprises, and the influence on enterprises that have not been included in carbon trading but may be included in the future is also very significant. It is also found that the innovation behavior of enterprises is not affected by the price of carbon trading, but by the way of quota allocation. This series of research findings provide a reference for the construction of China’s national carbon market.

Associate Professor Zhu Junming from the School of Public Administration of Tsinghua University is the first author and co-author of the article, while Deng Xinghua, a former postdoctoral fellow in Tsinghua University and now a lecturer at Southwestern University of Finance and Economics, is the co-author of the article, and Professor Xue Lan and Fan Yichun, an undergraduate graduate of Tsinghua University and now a master of Massachusetts Institute of Technology, are the co-authors of the paper.

Paper link:

https://www.nature.com/articles/s41467-019-12213-6

Zhong Yi’s research group of Tsinghua Institute of Life reveals the downstream molecular mechanism of active forgetting of different memory components.

On September 5th, the research group of Zhong Yi, School of Life Sciences, Tsinghua University published a research paper entitled "Genetic Dissection of Active Forgetting in Labile and Consolidated Memories in Drosophila" in PNAS.

In this paper, the downstream molecular mechanisms of active forgetting of unstable short memory and stable long memory in Drosophila were systematically compared and analyzed for the first time. It was found that the active forgetting of unstable short memory was mediated by Rac1/WAVE complex/ Dia pathway, while the active forgetting of stable long memory was mediated by Cdc42/WASp/ Arp2/3 complex pathway. As Dia and Arp2/3 complex are reported to regulate the linear growth and branching growth of neurofilament skeleton respectively, the research results of this paper provide an interesting possibility: the active forgetting of memory components with different stability may be related to the growth mode of different forms of neurofilament structure.

▲ Analysis of forgetting mechanism of stable memory and unstable memory.

As early as 1885, Hermann Ebbinghaus, a German psychologist, described the forgetting curve of the human brain for newly acquired memories, and began the journey of human beings to explore the mystery of forgetting. In the following 100 years, many scholars tried to find the mechanism of forgetting by using the method of experimental psychology, and also created a variety of theories to explain the phenomenon of forgetting, but ultimately failed to get a unified answer. As a basic feature of human memory, forgetfulness has been widely studied and reported in psychology, but almost no one cares about it in the research of neurobiology, and it has become a "forgotten corner". The main reason may be that the forgetting process is simply regarded as another description of memory failure, and it is a passive process (if you remember poorly, you will forget quickly, and if you remember well, you will forget slowly), thus ignoring the unique molecular mechanism of forgetting itself.

Until 2010, Professor Zhong Yi’s research group discovered the biological mechanism of active forgetting for the first time, that is, the learning process itself will activate the molecular pathway of forgetting signals to actively forget the formed memories. This active forgetting mechanism does not affect the quality of learning itself, but specifically affects the speed of forgetting acquired memories. Moreover, different memory components will have their own active forgetting mechanisms. For example, in the olfactory punishment learning of Drosophila, one training (5 minutes) can produce two completely different memory components at the same time: unstable anesthesia-sensitive memory (ASM) and relatively stable anesthesia-resistant memory (ARM) which lasts for more than one day. Zhong Yi’s research group has found that a study session can activate Rac1 molecule in the small G protein family to erase the unstable anesthetic sensitive memory that lasts for several hours, and activate Cdc42 molecule, another member of the small G protein family, to forget the more stable anti-anesthetic memory that lasts for more than one day. However, the specific downstream molecular pathways of the forgetting mechanism mediated by Rac1 and Cdc42 are not clear.

Recently, the research paper published online by Zhong Yi’s research group analyzed the downstream molecular pathways of these two forgetting mechanisms for the first time: Rac1/WAVE complex/ Dia pathway mediates the active forgetting of short-term memory components (unstable anesthesia-sensitive memories that last for several hours), while Cdc42/WASp/ Arp2/3 complex pathway mediates the active forgetting of long-term memory components (relatively stable anti-anesthesia memories that last for more than one day). Both of these active forgetting mechanisms occur in the mushroom body of the learning and memory center of Drosophila (the mushroom body contains about 4000 neurons, and the whole brain of Drosophila has more than 100 thousand neurons). Interestingly, cell biology research found that Dia was directly responsible for the linear growth of microfilament skeleton, while Arp2/3 complex was responsible for the branching growth of microfilament skeleton. This suggests that the active forgetting function of different memory components may be related to different forms of dynamic changes in the microfilament skeleton structure of neurons. On the one hand, these research results bring the academic understanding of the molecular mechanism of forgetting into a more detailed dimension, on the other hand, they provide more effective pharmacological targets for directly regulating the function of forgetting.

Gao Yang, a Ph.D. student in the School of Life Sciences of Tsinghua University, Shuai Yichun, a researcher in the Janelia Research Campus of the United States, Zhang Xuchen, a graduate doctoral student in the School of Life Sciences of Tsinghua University, and Peng Yuwei, a doctoral student, are the co-first authors of this paper. Professor Zhong Yi from Tsinghua University and Assistant Researcher Li Gan are co-authors of this article.

Paper link:

https://doi.org/10.1073/pnas.1903763116

Xiao Bailong of Tsinghua Pharmaceutical College and Li Xueming’s research group of School of Life jointly published a paper in Nature.

Reveal the delicate molecular machines structure and mechanism that mediates human tactile perception.

On August 21, Nature (Nature) The journal published the research paper "Structure and Mechanizing of the Mammalian Tactical Channel Piezo2" written by Xiao Bailong’s research group of Tsinghua University Pharmaceutical College and Li Xueming’s research group of College of Life Sciences. The three-dimensional structure and exquisite working mechanism of high-resolution cryoelectron microscope, a mechanical force molecular receptor-PIEZO 2 ion channel, which gives human tactile perception ability, are reported for the first time. This research is another important research achievement in this field after the two research groups reported in Nature on January 22nd, 2018 the high-resolution three-dimensional structure and molecular mechanism of another member of the mechanically gated PIZO channel family-PIZO 1 ion channel, which not only strongly promoted the understanding of the structural basis and molecular mechanism of PIZO channel family, but also laid a solid foundation for drug development based on PIZO channel. At the same time, Nature published a commentator’s article and spoke highly of the research.

Touch, as one of the five senses, not only gives us the ability to perceive pleasant touches such as shaking hands, stroking and kissing to maintain normal social behavior, but also bears the biological basis for human beings to skillfully use various tools such as touch screen, mobile phone or mouse. However, under pathological conditions such as tissue injury or inflammation, abnormal tactile perception function can lead to severe mechanical hypersensitivity (also known as touch pain). For example, patients with cancer or arthritis often suffer from severe pain caused by slight touch such as dressing or walking, which seriously affects individual health and quality of life. In addition, autistic people usually show more sensitive tactile perception than ordinary people. Recent studies suggest that this abnormal tactile function may be one of the important causes of autism.

Tactile perception originates from the response of mechanical force sensing molecular receptor-mechanically gated cation channel expressed on primary sensory neurons to mechanical force stimulation, which causes extracellular cations, such as sodium ions and calcium ions, to flow into cells, which in turn induces nerve cell excitement and signal transmission, and finally leads to tactile sensation. In 2010, the research group of Professor Ardem Patapoutian of Scripps Institute in the United States identified that the Piezo gene family encodes the necessary components of mechanically gated cation channels in mammals. In 2012, Dr. Xiao Bailong, who is engaged in postdoctoral research in Professor Patapoutian’s research group, and his colleagues reported in the journal Nature that Piezo protein constitutes the core pore component of mechanically gated cation channels, thus establishing a brand-new ion channel family type of mechanically gated Piezo channels for the first time.

Subsequent studies have proved that Piezo2 mediates the mechanical perception of tactile, proprioception (such as posture balance perception) and visceral perception (such as lung contraction and expansion, blood pressure perception and heart rate regulation) in mammals, while Piezo1 has been found to act as a molecular receptor of mechanical force in a variety of cell tissues and participate in regulating the development of blood vessels and lymphatic vessels, blood pressure homeostasis, bone formation and remodeling and many other functions. The genetic mutation of Piezo gene has been found to cause many human genetic diseases, including erythrocytopenia syndrome, lymphatic edema, distal joint contracture, tactile loss and so on. The human body with the loss-of-function mutation of Piezo2 not only shows tactile and proprioception defects, but also loses the perception of mechanical hypersensitivity in pathological state, which confirms that Piezo2 channel can be used as an important target for developing new analgesic drugs.

▲ Figure A, schematic diagram of frozen electron microscope of Piezo2, in which each subunit is represented by a different color; B, a schematic diagram of a dome-shaped structure surrounded by three outward twisted transmembrane paddle areas, and the transmembrane area is marked by the green dotted line; C, schematic diagram of the topological structure of Piezo2 with 38 transmembrane regions; D, a subunit structure of Piezo2 constitutes a display diagram; E, Piezo2 central pore module area; F, the central pore area of Piezo2 and Piezo1 surrounded by IH-CTD; The radius distribution diagram of the central pore area of G, Piezo2 and Piezo1, including the switch gate of transmembrane area and the Constriction neck)； of intracellular area; H, Piezo1 and Piezo2 mutants with deletion of hat region lack the current induced by mechanical stimulation.

Dr. Xiao Bailong’s research group comprehensively uses the multi-disciplinary research methods, such as biochemical structure, electrophysiological patch clamp, Qualcomm dosage drug screening, transgenic mouse model and human genetics, and focuses on solving the key scientific problems of how the mechanically gated Piezo channel converts mechanical stimulation into electrochemical signals, and how it uses its own mechanical sensitivity and channel characteristics to determine related physiological and pathological functions, and is committed to developing new drugs and technologies targeting Piezo channel. Up to now, as a correspondent (including co-author), he has made a series of important research achievements in the three-dimensional structure analysis, molecular mechanism revelation, small molecule drug discovery and physiological and pathological function exploration of Piezo channel.

In this latest Nature paper, relying on the technical system and research platform established by two research groups for analyzing the structure of Piezo1 channel by cryo-electron microscope, the researchers have made unremitting efforts for more than six years to overcome the difficulty of extremely low expression of mouse-derived Piezo2 with a total length of 2,822 amino acids. Through continuous exploration and optimization of protein purification conditions and sample preparation methods by cryo-electron microscope, a stable and homogeneous protein sample was finally obtained for data collection by cryo-electron microscope. In the process of structural analysis, Piezo protein is extremely flexible, which brings great challenges to the resolution of high-resolution structures. The researchers expanded the symmetry of Piezo2 data particles, cut them into three parts, and calculated them independently, so as to obtain high-resolution structures of each part and then put them together into a complete structure, overcoming the flexibility problem, and finally obtained a three-dimensional structure with an overall resolution of 3.6-3.8 angstroms (Figure A), and successfully analyzed the complete topological structure of Piezo2 protein including 38 transmembrane helical regions (Figures C and D).

The researchers found that the overall three-dimensional structure of Piezo2 channel is similar to that of Piezo1 channel previously analyzed, and it is assembled into a trilobal propeller-like structure with 114 transmembrane helical regions in the form of homotrimer (Figure a-d), and it is established that Piezo channel is a kind of large membrane protein with the most transmembrane times so far. Among them, the 1st to 36th transmembrane helix regions (TM1-36) are sequentially assembled into 9 repetitive structural units with the 4th transmembrane helix region as the unit, and this characteristic structural unit was previously named THU (Transmembrane Helical Unit) with the abbreviation of Tsinghua University (Figure c, d). Very interestingly, these nine thus are connected in series to form a special non-planar transmembrane "Blade" structure, which leads to the three blades enclosing into a dome-shaped structure with a diameter of 28nm and a depth of 10nm (Figure B). Researchers speculate that this dome-shaped PIZO protein-cell membrane system may be one of the structural foundations of PIZO channel with high mechanical sensitivity.

The extracellular "Cap" domain located above the central pore region is completely embedded in the bottom of the dome (Figures A and B), while there is a 9nm long Beam structure inside the cell, which connects the peripheral end of the blade to the intracellular position of the central pore region (Figures a-e). The 38th transmembrane helix (named inner helix, IH) and the trailing intracellular carboxyl terminal domain (CTD) form a central pore part (Figures E and F), including a narrow neck located in the transmembrane region and cytoplasmic region (Figures F and G), suggesting that the Piezo channel structure is in a closed state. The structural comparison between Piezo2 and Piezo1 shows that the transmembrane region narrow site is completely closed in Piezo2 structure, but open in Piezo1 (Figure f, g). Based on this, the researcher proposed that the narrow site forms a switch gate of transmembrane region (Figure f, g). Further structural analysis and electrophysiological function experiments show that the gate may be controlled by the rotation of the top hat domain (Figure H). However, because the narrow constriction neck located in the cytoplasm region is in the closed state in both Piezo1 and Piezo2 structures (Figure f, g), it remains to be further proved whether this site is controlled by the conformational changes of the blade and long rod structure as another switch gate.

Dr. Xiao Bailong said that through the full cooperation with Dr. Li Xueming’s research group, the analysis of the structure from Piezo1 to Piezo2 not only strongly promoted the understanding of the structure and mechanism of mechanical gating of Piezo channel in mammals, but also provided a solid foundation for exploring the mechanism of human diseases caused by the dysfunction of Piezo channel and the identification and discovery of related drugs. Relying on the all-round research platform and academic accumulation of Piezo channel established by their research group, they will devote themselves to the drug discovery and development of Piezo channel.

Dr. Xiao Bailong from Tsinghua University College of Pharmacy and Dr. Li Xueming from College of Life Sciences are co-authors of this paper. Wang Li, a postdoctoral fellow at Tsinghua University College of Pharmacy, Zhou Heng, a 2015 doctoral student at School of Life, Zhang Mingmin, a 2013 doctoral student, and Liu Wenhao, a 2016 doctoral student, are the first authors. In addition, Dr. Deng Tuan, Zhao Qiancheng (now a postdoctoral fellow at Yale University) and Li Yiran of Xiao Bailong’s research group also participated in some research work. Dr lei Jianlin of Tsinghua University cryo-electron microscope platform provided help for data collection of cryo-electron microscope.

Paper link:

https://www.nature.com/articles/s41586-019-1505-8.pdf

Graphic | School of Medicine, School of Life, Department of Electronics, School of Public Administration, School of Pharmacy

Editor | Li Jing

"Fashion is the most fashionable"　

Salute: as exquisite as Bond? Make costumes into high-end customized brands.

　 　 Kingsman is adapted from a cartoon of the same name in mark millar, which mainly tells the story of Harry Hart, an old agent of Kingsman Secret Service played by colin firth, training Egsey, a little agent played by taron egerton. However, Harry didn’t teach Egsey any real skills. It’s all up to Merlin, the bald instructor played by mark strong. Harry’s task, to be elegant, is to help Egsey, a street thug, become an elegant aristocrat, and to put it bluntly, to teach him how to dress. Therefore, the biggest attraction in the first half of this film is not fists or high-tech weapons, but Harry keeps showing off his suits, shoes, ties, watches and glasses. "Oxford is better than Brock", this clever trick of wearing shoes is an admonition that Harry emphasized to Egsey the most times. I think I was influenced by the spy film ipcress file starring michael caine in 1965.

　 　　Just like Bond, those clothes in Kingsman are well-known. In fact, the Kingsman Secret Service is ostensibly a tailor’s shop, located in the famous Saville Street in London. The shops in this street are all top clothing stores that serve the royal families and nobles of various countries. The prototype of Kingsman storefront is actually a world-famous custom clothing store Huntsman. It is worth mentioning that the film hired Ariane Phillips, who once served "Single Man", as a fashion designer, and cooperated with Mr. Porter, a British men’s fashion e-commerce, to create a customized series of "costume to collection" inspired by movies, which was sold in real life. In addition, the luggage accessories in the film are also of great origin: Turnbull & Asser’s shirt, Drake’s tie, Culture&Gross’s glasses, Swaine Adeney Brigg’s suitcase, Bremont’s watch and George Cleverley’s shoes.

Subversion: gentlemen don’t need muscle blocks!

　 　　Colin firth once played a homosexual in Single Man, and his performance style inspired Matthew Vaughn. We can hardly see any masculine flavor in Harry Hart, but we can experience a different kind of feminine charm. It is not difficult to understand that Matthew Vaughn is deliberately playing against Bourne ultimatum, Mission Impossible and the daniel craig version of 007. "Now spy films are getting more and more boring" is exactly what he thinks of these films. It doesn’t matter to him whether he has a muscle mass or not. The important thing is to wear a suit, which is the kind of elegance and grace shown between gestures. What he wants to pay tribute to is "Underwater City of 007" starring roger moore in 1977. Although this version of Bond has limited physical fitness, it has become the most attractive Bond in Vaughan’s mind because of its ease of weight and humor.

[Carrying equipment can kick and hit]

Salute: Which agent didn’t order high-tech goods?　

　 　　Harry is naturally very proud of his image and clothes, but Egsey, who just came out of the slum, doesn’t buy it. He grew up on the street and likes to wear hoodies or sportswear with hip-hop style. The rebellious teenager was easily fooled by Harry to report to the Secret Service, mainly because Harry’s cool weapons and cool skills shocked him. Like most spy movies, Harry’s high-tech equipment is enough to make him blind. The umbrella he carried when he first met him is a powerful weapon that can be attacked and defended. The tip of the umbrella can fire and the tarpaulin can prevent guns. And almost every accessory on him is a weapon at the same time. There is a sharp knife hidden in the toe of leather shoes, a 500,000 Ford catapult hidden under the dial of the watch, a miniature explosive with the power equivalent to a Grenade in the lighter body, and a toxic pill in the pen tube. Of course, his haute couture suit is also made of special bulletproof material.

Subversion: the moves are fierce and the hair can’t be messy.

　 　 When it comes to fighting, Harry’s action features are bright and neat, which can not only deal a powerful blow to the enemy, but also maintain the elegance of his posture and ensure that his carefully managed hairstyle will not be messed up. It was also in the scene where they first met Egsey that they met a gang of gangsters to provoke them. Harry calmly closed the doors and windows of the hotel, knocked down the gangsters one by one with three strokes and five divisions, and it was like a dance. Colin firth trained for three hours every day for several weeks in order to make a good show. The crew specially invited a number of experts to help, including Jackie Chan’s training team, world Muay Thai champion and Olympic gold medal gymnast. There is also a special forces guy who is responsible for guiding how to use a gun.

　 　　However, if you really meet a strong opponent, it is out of place to consider the image of a gentleman. Terrorists who intend to cleanse mankind are all ruthless dissidents, and agents can only protect themselves if they give them back in their own way. Vaughn deliberately designed the action scene to be fierce and abnormal, which led to the final R rating of the film. Take the opening scene as an example. Agent Lancelot went deep into the tiger’s den to save the hostages and killed all the terrorists. He was so ruthless that people thought he was a villain. Unexpectedly, in the blink of an eye, the goods were split in half by the female killer Jia Zeiler (sofia boutella). Cruel enough! That’s not all. Vaughn later let this sad agent appear again, and his two halves of the body were sewn together by a rope for the villain Valentine (samuel jackson) to "enjoy and ponder". Such humor may be the essence of Vaughan’s aesthetics.

　　"Ningbo released" the news of WeChat official account on September 14th. In order to make overall plans for normalized epidemic prevention and control and typhoon prevention and flood control, the Municipal Epidemic Prevention and Control Office issued the latest notice with the consent of the Municipal Leading Group for Epidemic Prevention and Control.

　　01

　　Due to the impact of Typhoon Meihua, on September 15th and 16th, the city continued to implement the current nucleic acid inspection requirements except the airport, railway station, high-speed bayonet, high-speed service area and other city "gates", and the city continued to suspend the 72-hour normalized nucleic acid inspection requirements. If the nucleic acid test shows "gray code" for more than 72 hours, citizens can still take public transportation and enter public places, but please continue to cooperate with the staff to check the health code and travel code.

　　02

　　Since September 15th, all localities should restore and open normalized nucleic acid sampling points in an orderly manner, increase sampling units, extend sampling time, and effectively protect the needs of citizens for nucleic acid sampling. All districts (counties, cities) should promptly publish information such as sampling site and opening hours of nucleic acids.

　　03

　　From 0: 00 on September 17th, Ningbo will fully resume the 72-hour normalized nucleic acid detection and inspection requirements implemented on September 7th. Please understand, support and cooperate with the general public, and please tell each other.

CCTV News:Five departments, including the Ministry of Industry and Information Technology, issued an announcement on matters related to the implementation of the national six emission standards for automobiles.

In order to implement the Emission Limits and Measurement Methods of Light Vehicle Pollutants (China Phase VI) (GB 18352.6— 2016) and "Pollutant Emission Limits and Measurement Methods for Heavy Diesel Vehicles (China Phase VI)" (GB 17691— In 2018), the relevant policies to help enterprises bail out, stabilize and expand automobile consumption are implemented. The relevant matters concerning the nationwide full implementation of the National Sixth Emission Standard 6b for light vehicles and the National Sixth Emission Standard 6b for heavy diesel vehicles (hereinafter referred to as the National Sixth Emission Standard 6b) are announced as follows:

1. From July 1, 2023, the national sixth emission standard 6b stage will be fully implemented, and the production, import and sale of automobiles that do not meet the national sixth emission standard 6b stage will be prohibited. The production date is based on the vehicle manufacturing date of the motor vehicle certificate, and the electronic information of the certificate should be uploaded before 0: 00 on July 1, 2023; The date of import is based on the date of arrival of the goods import certificate; The date of sale shall be subject to the date of motor vehicle sales invoice.

Two, for some of the actual driving pollutant emission tests (that is, RDE test) report results as "monitoring only" and other light vehicles, give a half-year sales transition period, allowing sales until December 31, 2023.

Three, automobile production and import enterprises, as the main body responsible for the consistency management of environmental protection production, should disclose the vehicle emission inspection information and pollution control technical information before the vehicle leaves the factory or enters the country according to the relevant provisions of the People’s Republic of China (PRC) Air Pollution Prevention Law, so as to ensure that the actually produced and imported vehicles meet the requirements. Relevant certification bodies shall issue compulsory product certification certificates according to the national six emission standards 6b stage.

　　Recently, a master’s thesis entitled "Sociological Research on Fart" was circulated on the Internet, causing controversy. Some netizens are "sarcastic": Cold Window has studied hard for so many years, and it is really a "fart". Some netizens commented after reading that things that seem meaningless and are sneered at are "serious things to dig into."

　　The Paper noted that the paper was written in 2007 by Gao Jianwei, an in-service master of sociology at Huazhong Normal University. At that time, Gao Jianwei not only passed the master’s thesis defense with this paper, but also was rated as "excellent".

　　As early as 2015, the paper had aroused public concern. Li Yaxiong, a tutor of Gao Jianwei and a professor at Huazhong Normal University, said in an interview with the media at that time, "There are actually many aspects in life that are worth exploring, and there is no need to always repeat some meaningless topics. Some topics that look different or niche are often more worthy of consideration and there will be more interesting discoveries."

　　In this regard, Zheng Zuoyu, a professor at the School of Sociology of Huazhong University of Science and Technology, believes that whether the paper can be written well depends on whether the attitude is correct. In his view, even if the topic is high, but the attitude is not good, "no use"; On the contrary, if the academic attitude is correct, the topic "No matter how bizarre it is" can also be described as a classic.

　　The thesis was praised by the tutor.

　　"I am often mentioned by people around me such a question: How did you do such a topic?" Gao Jianwei explained at the beginning of his thesis "Sociological Research on Fart" that he chose this topic because he accidentally saw two stories about fart on the Internet. In both stories, the protagonist changed his life track because of a fart, which triggered Gao Jianwei’s thinking.

　　Gao Jianwei believes that "fart" is not only a physiological phenomenon, but also a social phenomenon, which has various correspondences with social relations. Through the study of a large number of historical materials, he pointed out that fart exists as a social taboo in both western culture and eastern culture. This taboo not only spreads in all aspects of our daily life, but also widely exists in the superstructure fields such as education, manners and customs, habits, morality and even law.

　　Gao Jianwei deeply analyzed how fart behavior and phenomenon changed from a physiological behavior to a social taboo from the perspective of social genetics, and revealed the complex mechanism of this operation process. In the process of social development, fart has evolved from indiscriminate human behavior to behavior that occurs more frequently among some social members, and then to negative behavior in moral evaluation, and finally evolved into a form of social taboo.

　　The paper mentions that in the early stage of human beings, division of labor and class have not yet appeared, and fart behavior is primitive human beings — — That is, the most frequent and common physiological phenomenon of our ancestors. With the development of productive forces, social relations have changed, and the diet types and habits of the rich have changed, and they no longer fart frequently. At this point, the human fart experience has completed the first historic change, that is, from a common physiological phenomenon of human beings to a behavior that occurs more frequently and more commonly among some members of society (more poor people).

　　Through this transformation, fart behavior has gained some social significance, reflecting the fact that the upper and lower classes coexist and oppose each other. In order to maintain its dominant position, the upper class not only establishes an absolute control position in the political, economic and cultural fields, but also strives to "shape" the social state that adapts to its dominant position. Distinguish the upper and lower classes by etiquette, self-cultivation and taste. The upper class is often considered polite, while the members of the lower class are ordinary, vulgar and even despicable. In this context, farting is labeled as a negative moral label. Under the power of discipline, "fart" has changed from a negative social behavior defined by the upper class to a social taboo, and has been recognized and obeyed by all members of society.

　　He believes that this genetic process comes from the strange characteristics of power operation, namely, duality, dependence and publicity. Then it discusses the fart behavior and phenomenon of "no taboo", and thinks that this phenomenon essentially reflects the taboo existing form of fart behavior and phenomenon, and is related to the strange characteristics of power operation.

　　The Paper noted that this is not the first time that this paper has been concerned by netizens. In 2015, Beijing Evening News reported on this matter. Li Yaxiong, Gao Jianwei’s tutor and professor of Huazhong Normal University, was quite appreciative of the paper when interviewed.

　　According to his memory, when the paper was opened, the teachers all laughed at the first sight of the topic, but after the "laugh", everyone quickly discussed its academic value from a professional point of view, not simply talking about the matter, but using sociological theory for analysis. Li Yaxiong told the Beijing Evening News reporter at that time that Gao Jianwei’s research found a new way. In the case of repeated research, it was "precious" and teachers generally supported it.

　　"The defense effect is also very good, and it has also been rated as excellent. He (Gao Jianwei) has a good writing style and clear thinking. " Li Yaxiong said that because it is not convenient to do actual investigation, Gao Jianwei presented the genesis of fart, its vertical development in different times and the social meaning behind it through literature analysis and quotations. "I welcome this topic that comes from life and has humanistic care."

　　In this regard, The Paper recently contacted Professor Li Yaxiong several times for an interview, but the other party declined on the grounds of being away on business.

　　College teachers: With a correct attitude, no matter how strange the topic is, it can become a classic.

　　In fact, in recent years, many papers have attracted public attention because of their unique topics. Previously, papers such as "The Utopian Cat: The Identity and Fantasy of Cats Fans" and "The Influence of Illicium verum on the Volatile Flavor of Braised Chicken and Its Mechanism" have caused heated discussions.

　　"This is a question of diversification of topics, which I think is a good thing." Rui Liu, tutor of master students in Huazhong University of Science and Technology, believes that the development of disciplines is characterized by "specialization" and "cross-integration", and "many problems that we didn’t think could be studied before have emerged now". "Personally, I think it is necessary to study such different problems, which can cover many blind spots of previous knowledge and inspire the society." Rui Liu said in an interview with The Paper recently.

　　In this regard, Zhou Tingting, an associate professor at the School of Journalism, Huazhong University of Science and Technology, believes that college students are active in thinking or familiar with new media, so they can really pay attention to some new phenomena. "Many phenomena can be used as the research topics of our thesis, but we should find an in-depth observation angle based on the discipline, not just describe ‘ Phenomenon ’ It is meaningless to do a simple analysis. " Zhou Tingting said.

　　Tan Songhua, a member of the National Education Advisory Committee, said that he had not read the above papers and could not comment on them. "How to write a graduation thesis, it is difficult to have a unified statement. Students with different majors, hobbies and energies have different factors to consider when choosing a topic. " Tan Songhua said that as far as he guides graduate students, the practical significance is more important when choosing the topic of the thesis, for example, it can promote the development, reform and social progress of the country.

　　For more and more "unique topics", Zheng Zuoyu, a professor at the School of Sociology of Huazhong University of Science and Technology, said that "there is nothing wrong with it". "Academic research should have cared about your life and the society you live in. Now that the era in which students live is full of these phenomena, it is natural to study these phenomena. " According to Zheng Zuoyu, the question is not whether the research topic is "innovative" or "serious", but whether the research attitude is correct.

　　Zheng Zuoyu said that individual students "lead a muddled life" and "choose the most mindless topic" when writing their papers. "Such a topic, even if it sounds tall, is actually vague." Zheng Zuoyu said that attitude is more important for writing papers.

　　"When I take part in the insurance research interview, I will ask students what fields they are interested in and have studied." Zheng Zuoyu found that most students from rural areas would say "I want to do rural research", or most female students would say "I want to do gender research". "But not many of these students have really studied the relevant knowledge. They just think that because of their identity, they can make some sense at worst. " Zheng Zuoyu believes that in this case, even if the topic is tall, the attitude is not correct, and it is "no use"; On the contrary, if the academic attitude is correct, the topic "No matter how bizarre it is" can also be described as a classic.

　　What do students think about the topic of the thesis? Li Yong (pseudonym), a graduate student in a university in Hunan, told The Paper that according to his observation, the source of the topic mainly has three parts, one is the combination of previous practical activities, the other is the combination of interests, and the third is from the tutor. "I prefer topics that I am interested in, and at the same time consider the significance of research, so I am motivated to write." Li Yong believes that interest is a good entry point. "When the school informs you to write a paper, you often feel confused and then choose a familiar field." (The Paper reporter He Liquan intern Hu Tianyi)

Original six-story demon tower

Photo: Huang Zi | Written by: Six-story Demon Tower | Editor: Ayue

▲ Image source: homemade in the eleventh clinic

Have not had sex all the time, can you not do gynecological examination?

First of all, answer the first question: even if you have never had sex, you need to have a gynecological examination.

Of course, here we should distinguish between broad and narrow gynecological examinations. When it comes to gynecological examination, many fairies first associate it with gynecological examination.

It requires women to lie on a special gynecological examination bed, and doctors can open the yin channel through a speculum (duck-billed forceps) to check the yin channel and the cervix, and if necessary, take samples and check the secretion of the yin channel and the exfoliated cells of the cervix, and then know whether there is any abnormality in the uterus and bilateral ovarian fallopian tubes through Shuang Hezhen or triple diagnosis.

The gynecological examination that our doctors usually say includes a series of laboratory tests such as ultrasound and tumor markers, in addition to physical examination.

For female friends who have never had sex, gynecological examination is definitely not suitable, but this does not mean that we don’t need gynecological examination.

We should not actively ignore or passively refuse gynecological examination because we have never had sex.

Yaota met many female patients who had never had sexual life in clinical work. It was precisely because of the wrong idea that there would be no gynecological diseases without sexual life that the best treatment opportunity for the disease was delayed. Among them, there were many cases of malignant tumors, which can be said to be a lesson of blood.

What makes me feel most sorry is a patient not long ago. She never had sex, so she never had a gynecological examination. She had a gynecological B-ultrasound by chance and found a tumor in her uterus. She sought some Chinese medicine treatment by mistake, but it didn’t work. One day she came to our hospital because of acute abdominal pain.

We thought it was just a simple fibroid degeneration, but after the operation, we realized that the condition was far more serious than we thought. The final postoperative pathology was uterine sarcoma, a highly malignant uterine tumor.

After surviving the postoperative rehabilitation period and unusually uncomfortable chemotherapy, I thought everything was going well. However, less than a month after the end of chemotherapy, the tumor recurred and metastasized to the intestine, and she underwent laparotomy again.

The last time I contacted her, she told me, Yaota, I won’t go to your hospital. The tumor has metastasized to the lungs. I’ll go to another hospital. Later, I didn’t see her update her circle of friends. I don’t know how she is now, but I can still remember her strong smile.

▲ Image source: giphy

Forgive the demon tower. I’m old, and I feel it. It’s a bit far.

What tests should I do if I have no sex life?

Then answer the second question: even if we have never had sex, we can still do gynecological B-ultrasound (transabdominal or anal ultrasound); You can also check yin secretion, tumor markers and hormone levels. And from the point of view of gynecological physical examination, although we can’t do Shuang Hezhen, we can learn about pelvic cavity through anal examination when necessary.

Gynecological B-ultrasound: It can help us to know the thickness of endometrium, whether there is abnormal echo in uterine cavity (such as endometrial polyp and submucosal myoma), whether there is abnormal echo in myometrium (such as hysteromyoma and adenomyosis) and whether there is abnormal echo in ovarian fallopian tube (such as ovarian tumor and hydrosalpinx);

Yin secretion: it is not that there will be no yin inflammation without sexual life! Doctors can use a cotton swab to get yin secretion for laboratory examination without opening the yin channel with a speculum.

Tumor marker: This test is more routine and simple, that is, blood drawing. Among many tumor markers, CA125, which is closely related to female diseases, is the most common and you know the most. Generally speaking, CA125 will be elevated in many cases, such as before and after menstruation, endometriosis, adenomyosis, pelvic inflammatory disease and ovarian malignant tumor.

Hormone level: including six items of sex hormones, AMH and other tests, we often need to use the results of six items of sex hormones to help doctors judge the condition when diagnosing and treating female endocrine diseases such as abnormal uterine bleeding and polycystic ovary. AMH can help us understand the reserve function of female ovaries and better guide pregnancy preparation. These are also blood tests.

▲ Image source: giphy

What will happen if I accidentally do the yin examination?

The third question: What will happen if you accidentally do the yin examination?

Before answering this question, first of all, we need to be clear. First, we can’t choose not to check out because of fear or concern. Second, when we ask the doctor, we should clearly tell the doctor that I have never had sex before, so as to avoid the possibility of accidentally doing yin examination to a great extent.

Through the description of gynecological physical examination in Yaota, I believe you may have guessed that the following may happen to women who have no sexual life after yin examination.

ache

Yin Dao flap (common name hymen) is a mucous membrane with certain elasticity. With the invasion of speculum, yin Dao flap has not broken yet, but it will induce vulvar pain due to expansion. At this time, something must be done to stop it.

Rupture and bleeding of yin valve

Some female friends have high elasticity of the yin valve, and there may be no obvious pain in the process of entering the speculum, but with the opening of the upper and lower leaves of the speculum, the yin valve will basically rupture or even bleed during this process.

At this time, it is necessary to compress the wound to stop bleeding. Generally, a sterile cotton ball will be selected, and it will be removed after 5 to 10 minutes of compression to observe the bleeding of the wound.

Because the yin flap is thin, there are often some capillaries distributed here, which can basically stop bleeding through compression. However, if it happens to damage a relatively large blood vessel, it may be necessary to stop bleeding through suture if necessary, but this situation is very rare.

infect

If this happens unfortunately, you must pay attention to the cleanliness of the vulva after the bleeding stops, so as to avoid infection of the wound. Of course, this kind of situation is also very rare, because this kind of wound of yin mucosa usually heals very quickly.

Yao ta Xiao tips

If the doctor gives you a gynecological B-ultrasound checklist, you must see clearly whether it is through the yin channel, abdomen or anus before paying the fee. If it is through the yin channel, or if you feel that you can’t accept the transanal B-ultrasound examination, you must find a doctor to change the checklist.

Original title: "It is strongly recommended that all women must do these four tests during physical examination (whether they have sex or not)"

Read the original text

　　Some professional qualification certificates can be inquired through the national networking system.

　　Investigation on the phenomenon of illegal sale of "real certificate"

　　Investigation motive

　　It seems that the demand for various qualification certificates through informal channels has not decreased, which can be seen from a fraudulent case of manufacturing and selling fake certificates on the websites of state organs uncovered by the police in Nantong City, Jiangsu Province. In this case, the police have initially identified more than 34,000 pieces of information about people who handle false certificates, involving labor and personnel, science, education and culture, health care and medicine, engineering construction and other fields, and the personnel are distributed in more than 20 provinces, cities and regions across the country.

　　Where there is demand, there is market. From street poles to online shops, accreditation advertisements can be seen almost everywhere. In everyone’s impression, such advertisements for accreditation correspond to fake documents, just like the case cracked by Nantong police. However, the "Legal Daily" reporter found that in the accreditation business, some businesses claimed that they could provide "genuine" professional qualification certificates.

　　□ Our reporter Han Dandong

　　□ Intern Li Wenguan of this newspaper

　　Han Lin, who is studying at Tianjin Normal University, has been struggling recently. Seeing that she will graduate next year, she hasn’t got a teacher’s qualification certificate so far because Mandarin is not enough, which has a great impact on finding a job.

　　There are two choices before Han Lin, either practicing Putonghua hard and taking the exam again, or spending money to get a Putonghua grade certificate. "Compared with the two, the risks are the same." Han Lin said, "if you take the exam again, you may still fail;" Spend money to apply for a certificate, although the other party promises to inquire, but I don’t know whether it is true or not. "

　　Han Lin told the reporter of Legal Daily that it is almost an open secret among the students to spend money on the real certificate that can be inquired through official website. According to her understanding, in addition to the teacher qualification certificate, legal professional qualification certificate and other certificates can not be bought online, some professional qualification certificates, such as Putonghua grade certificate, health certificate, nutritionist and other certificates can be bought online, "it is said that it can also be found on official website".

　　According to the regulations, applicants need to take the corresponding exams and meet the standards before they can obtain the qualification certificate. Does it really exist that you can get a qualification certificate without taking an exam and be able to query through official website? The "Legal Daily" reporter launched an investigation on this.

　　3,500 yuan to run the Putonghua Grade Certificate.

　　For those who want to engage in the teaching profession, the Putonghua grade certificate is a necessary document. However, some people miss out on the teaching position because Mandarin is not up to standard, such as Zhou Wei.

　　Although I majored in education in college and always wanted to be a teacher, Zhou Wei has been unable to apply for a teacher position. "Because my Mandarin is not enough, I can’t take the teacher qualification exam, so I can’t apply for a teacher. Now I can only do some non-teaching work in some small training institutions."

　　Zhou Wei said that the failure of Putonghua has always bothered her, so she paid attention to all kinds of information about the Putonghua exam. At the beginning of this year, when she inquired about relevant information, she found that an online shop was selling Putonghua Grade Certificate, and it could be inquired through official website. "I chose a good online shop. The store said that 3,500 yuan can be used for a first-class and second-class certificate of Putonghua. Pay the deposit of 600 yuan first, and it can be done in two months. After another month, you can check online, and you can pay the final payment after the inquiry. " Zhou Wei said, "At that time, I tentatively asked if I could directly apply for a teacher qualification certificate. The store’s statement is that it is too difficult to apply for a teacher qualification certificate. "

　　Holding the psychology that "a dead horse is a living horse doctor", Zhou Wei paid the store a 600 yuan deposit. Two months later, the first-class and second-class certificate of Putonghua was sent to Zhou Wei. About a week later, the store told Zhou Wei that she could inquire. Zhou Wei logged into the national Putonghua training and testing information resource network and found that both scores and certificates could be found. Zhou Wei then clicked on the online store to confirm the payment.

　　With the experience of handling Putonghua Grade Certificate, Zhou Wei took advantage of the opportunity to spend money to buy a second-class computer certificate. "I checked, and there is no problem."

　　Online shopping level 4 chef’s certificate can be queried online.

　　Liu Jun, from Lanzhou City, Gansu Province, also told reporters about his experience of buying a certificate.

　　In July this year, Liu Jun learned from a friend that he could buy a certificate. After that, he searched for an online shop with a permit business on an e-commerce platform.

　　"I’m a good cook. I’ve always wanted to get a chef’s certificate. I just tried it that time." Liu Jun told reporters that after he told the online shop owner about the need for a chef’s certificate, the other party said that there was no problem, and he only needed to provide a 1-inch ID photo and a photo of the front and back of the ID card. He sent the photos to the other party’s mailbox as requested by the other party.

　　"The shopkeeper said that the first cooperation was a sign of sincerity, and payment could be made after the certificate was completed. It will take about two months to get the certificate. Official website needs to wait for a month or so after the certificate is completed. " Liu Jun said.

　　On September 21st, Liu Jun received a photo of the certificate from the owner of the online shop. "What is shown above is my personal information. The column of occupation (type of work) and grade is filled in as Chinese Cooker Level 4. The score of theoretical knowledge test is 67, and the score of operational skills test is 68, and the evaluation result is qualified." Liu Jun said that the certificate was issued on September 14th, 2017. At that time, official website still couldn’t find it. The owner said that online inquiry will have to wait for about a month.

　　On November 10th, Liu Jun received a notice from the owner that the previous certificate had been online. Liu Jun inquired online according to the information of the photos he had received before, and information such as personal basic information and test scores appeared on the webpage, which was exactly the same as the information on the certificate. The data was reported on November 7.

　　Subsequently, Liu Jun forwarded the previously agreed 400 yuan to the other party via WeChat, and sent the address to the other party. The owner said that it would be mailed as soon as possible and paid by SF Express. On November 13th, Liu Jun received the documents mailed by the other party.

　　Liu Jun showed the reporter this "real certificate" purchased from the Internet. The reporter saw that the official seal of the issuing authority on the left side of the certificate is the special seal for the appraisal of the Human Resources and Social Security Bureau of a city in Jiangxi Province, and the special seal for the professional skill appraisal of occupational skill testing authority of a city in Jiangxi Province on the right side of the certificate, with the words of steel seal clearly visible.

　　Customer service reminds me to search for official website query by myself.

　　Is Zhou Wei and Liu Jun’s experience of buying securities a case or a market?

　　On an e-commerce platform, the reporter of Legal Daily searched for the keyword "certificate", and thousands of products appeared in the query results. However, the query page says "training" and "certificate holster, diploma qualification certificate driver’s license shell … …”

　　"Legal Daily" reporter entered an online shop, which opened in 2009, with a favorable rate of more than 98%. The owner of the online shop wrote in the statement: "Chengzheng Studio undertakes all kinds of complicated certificates, five-star service, fair price, professional technology, advanced equipment, quick delivery and your satisfaction are the driving forces for us to continue. Welcome everyone to come! Registration process: consult and confirm the registration materials submitted for the registration course and pay the registration fee. After checking the certificate, pay the final payment SF mail certificate. "

　　The "Legal Daily" reporter opened the online consultation window of the store, and the other party directly asked: "What certificate do I need to apply for?" The reporter asked if he could apply for a welding certificate, and the customer service quickly replied "Yes" and introduced the price of each certificate to the reporter.

　　The "Legal Daily" reporter asked the store owner how to pay for all the treasures in the online store. The customer service said that after discussing the price of the certificate, just shoot the baby in the certificate shell directly. The price of the certificate shell is 10 yuan, so you can shoot more babies according to the certificate price.

　　Subsequently, the reporter of Legal Daily searched with "professional qualification certificate" as the key word, and there was no need to filter. The search results all read "professional qualification certificate, human resource manager level 1, level 2 and level 3". The reporter clicked into an online shop named "Yimei Culture and Education Center". Just entering the online store, the customer service staff asked the reporter what certificate he needed to apply for. The reporter asked if he could apply for a chef’s certificate. The customer service replied: "Yes, you need to provide one-inch or two-inch recent photos (blue or red background, white background) and a copy of your ID card. You can only apply for intermediate or advanced, intermediate 780 yuan, and advanced 1,280 yuan. The intermediate certificate will be issued in about two months, and the advanced certificate will be issued in three or four months. After the certificate is issued, it will be queried on the national website in two months."

　　The reporter asked how to ensure the authenticity of the documents. The customer service immediately said, "It’s impossible for a fake certificate to come down for that long. It’s good to make a fake certificate for a few days. Besides, you have to go to jail for making a fake certificate. If you don’t believe it, you can wait until you find it before confirming the receipt. "

　　After listening to the introduction of customer service, the reporter said that some inquiry websites are fake now. The customer service reminded the reporter that you should not use the website that the merchant gave you to inquire, but directly search for the national vocational qualification certificate from the Internet, and inquire from the regular official website. "The real certificates are all checked about two months after the issuance of the certificate, and the national inquiry system is updated regularly, so it is impossible to check the certificate."

　　Online shop trading cycle is consistent, but prices are different.

　　In an online shop named "Jiangxi Textual Research Network", the reporter of "Legal Daily" just clicked on the chat page, and the other party suggested that the reporter contact by phone or WeChat. The reporter added a WeChat according to the mobile phone number provided by the other party, and this is called "Jiangxi Textual Research Studio — — Teacher Li’ s people started to communicate. The reporter asked what documents he could apply for, and "Teacher Li" said that he could only apply for some professional qualification certificates, and guaranteed that they could be checked.

　　An online shop named "Macy’s University Hall" opened on October 11th, 2004, with a favorable rate of 99.44%. It is located in Nanjing, Jiangsu. When entering the store, there are three points for attention on the home page: special goods, once sold, will not be returned, please know; All trainees who have been trained by this institution shall ensure to get the vocational qualification certificate recognized by the state; All certificates can be checked for permanent registration, which can be checked in this life and in the next life. At the bottom of the page, the optional certificates include 10 categories, such as business services, electronic information and building electrician.

　　The "Legal Daily" reporter inquired about a number of online stores on an e-commerce platform. The shopkeepers’ caliber was almost the same, and they all guaranteed to issue certificates in two or three months. After issuing certificates, they could check online for two months. Most of them traded by paying the deposit first and then paying the final payment. The materials needed to apply for certificates are almost the same, mostly photos and copies of ID cards, and some need to fill out a form, including the name, age, education and other basic information of the applicant. The prices of these documents vary. Take the intermediate vocational qualification certificate as an example. Some merchants ask for three or four hundred yuan, while others ask for seven or eight hundred yuan, all of which promise to be checked for life.

　　Vocational skill appraisal institutions organize examinations.

　　According to the regulations, what are the procedures for applying for vocational qualification certificates?

　　According to a staff member of the Business Department of the Human Resources and Social Security Bureau of Lanzhou City, Gansu Province, citizens who want to obtain vocational qualification certificates should register at the vocational skill appraisal institute first. Vocational skill appraisal institute is established with the approval of personnel department, and every district in Lanzhou has vocational skill appraisal institute. After the registration, the vocational skill appraisal institute first examines the qualifications of candidates, including age, whether the education is appropriate, and the type of personnel, such as students or social workers. After the vocational skill appraisal institute examines the qualifications of the registered candidates, the Municipal People’s Social Security Bureau will conduct further qualification examination, mainly to see whether there are conditions for entering the examination. In the third step, the vocational skill appraisal institute needs to do preparatory work before the exam, such as printing the admission ticket. These are all audit links, and they are also the work that needs to be done in vocational skill appraisal. The fourth step is to organize the candidates’ examination by the vocational skill appraisal institute. After the candidates get the admission ticket, they will take the examination according to the time and place specified in the admission ticket. At the end of the exam, the vocational skill appraisal institute will score and score, and it will be qualified if it reaches 60 points or more. After that, the vocational skill appraisal institute reported the data of qualified candidates to the Municipal People’s Social Security Bureau. The Municipal People’s Social Security Bureau will further review various information such as candidates’ grades and ages. After the examination and approval by the Municipal People’s Social Security Bureau, the data of qualified candidates will be reported to the provincial appraisal center. After the final review, the provincial appraisal center will inform the Municipal People’s Social Security Bureau of the results, and one who is qualified will be given an empty certificate, and then the Municipal People’s Social Security Bureau will fill in the information and finally issue the certificate.

　　According to this staff member, the examination was organized by the Vocational Skills Appraisal Institute. During the examination, the Municipal People’s Social Security Bureau organized personnel to conduct on-site inspections. For the examination papers, the Municipal People’s Social Security Bureau should also organize personnel to conduct spot checks, and the workload of the census is too great.

　　So, what is the vocational skill appraisal institute?

　　The reporter inquired that both the vocational skill appraisal institute and the human resources service intermediary agency applied to the municipal human resources department or the provincial human resources department for establishment.

　　Article 69 of the Labor Law of People’s Republic of China (PRC) stipulates: The state determines the occupational classification, sets the vocational skill standards for the specified occupations, and implements the vocational qualification certificate system, and the assessment and appraisal institutions approved by the government are responsible for the assessment and appraisal of workers’ vocational skills.

　　Paragraph 2 of Article 26 of the Law of People’s Republic of China (PRC) Municipality on the Promotion of Private Education stipulates that students who have received vocational skills training and passed the appraisal by vocational skill appraisal institutions approved by the government may be issued with national vocational qualification certificates.

　　How to apply for the establishment of a vocational skill appraisal institution? The reporter inquired about the information and found that the conditions for accepting the identification of vocational skill appraisal institutions by local human and social departments are almost the same. Taking Tianjin as an example, the application conditions are as follows: vocational skill appraisal institutions should meet the basic conditions stipulated by national laws, regulations and policies; The sponsor shall have the legal person qualification; Having a clear name, organization and articles of association; There are full-time and part-time assessors; Having the purpose, objective and outline of appraisal; Equipment and facilities meet the prescribed standards; The registered capital reaches the prescribed standard; There are venues that meet the needs of identification.

　　(At the request of the interviewee, the interviewee in this article is a pseudonym.)

　　Cartography/Li Xiaojun

　　"If you are in politics, you will be governed by the feelings of the next, and if you can’t, you will be chaotic." An important aspect of keeping close contact with the masses is the trend of investigation and research in Daxing.

　　— — Speech by the Supreme Leader at the Party Building Work Conference of Central and State Organs (July 9, 2019)

　　"When you are in power, you will be governed by the emotions, but if you are not in power, you will be in chaos." The language Mozi Shang Tong means that politicians should fully grasp the emotions and public opinion before they can manage the society well, otherwise it will easily lead to chaos. To understand the next situation, we must sink down to the people in a down-to-earth manner.

　　In the Ming Dynasty, there was an official named Zhou Chen who liked to "go into the fields, talk to the villagers and ask about their sufferings". One night, Zhou Chen passed by a village and saw villagers enjoying the cool under the tree, so he took the initiative to talk. It was not until the subordinates found it that the villagers knew that the person in front of them was actually the governor of Jiangnan. In this way, "every time I sit in one place, I am afraid that it will not be exhausted." Zhou Chen learned a lot of local facts through face-to-face conversations with the people.

　　Later, Zhou Chen founded the "square meter law" and reduced the rent of grain from official land, which reduced the burden on the people and solved the problem of tax arrears. He insisted on consulting the people, and many reform strategies came from research. People commented: "Every time Zhou Wenxiang made a revolution, he would oppose reconsideration with officials and scholars. For example, if you elaborate a method, you will call Dongmen huangpo into the platform and often leave a message until midnight. "

　　"The feeling of winning" can not only know "the hardship of farming" and "the hardship of spinning performance", but also serve as an important basis for scientific decision-making. Historically, Shang Yang’s "a strong country knows thirteen numbers" and the two tax laws of the Tang Dynasty were all strategies for governing the country after in-depth investigation.

　　Wang Zhiwang, an official in the Southern Song Dynasty, was the first person who conducted a large-scale public opinion survey in ancient China and revised the national tax policy on this basis. At that time, in view of the uneven land tax and chaotic taxation, the Southern Song government decided to clear the land and rectify the taxation, and introduced the "Economic Boundary Law", but there were great differences among the people. Wang Zhiwang realized that "the matter is the most important, the opinions are different, the interests are suspicious, and I dare not judge by partiality." So, he began to make a comprehensive survey.

　　Wang Zhiwang asked his subordinates to go door-to-door to see how many people supported the old tax law and how many supported the new tax law, and then summarized and analyzed them. This time, a total of 9 States, more than 30 counties and more than 330,000 households were involved, and the amount of work was huge.

　　According to the investigation results, Wang Zhiwang revised the tax reform plan and submitted "On the Management of Tongchuan Road" to the court. In the memorial, he emphasized the different opinions of the people in different States on the "Jing Jie Law": "There are many people in the four States of Huai An Army in Changrong, and there are few people in Fushun Supervision in Suining, Tongchuan." This solid memorial provided a reliable basis for the improvement of the imperial policy.

　　Paying attention to observing people’s feelings and advocating investigation and research are important contents of China’s traditional political culture. In the Western Zhou Dynasty, poetry collectors went to various places to "observe customs and know gains and losses". Some people said that the Book of Songs was an ancient investigation report. Guan Zi Wen puts forward dozens of questions to be asked, which is considered as "the oldest and most comprehensive outline of social investigation". All dynasties have established various patrol systems, which play an important role in reaching the next level.

　　Compared with ancient times, today’s means of transportation and communication are more and more developed, and there are more and more channels to obtain information, but the convenience of technology also brings some problems. For example, some cadres would rather sit in the office and read all kinds of data and collect research reports on the Internet than go to the grass-roots level to dip their legs in mud and stand in the field to chat with the people. This kind of "paper" research can’t get the real situation at all, so how can we formulate policies on this basis?

　　Liu Xiang said in Shuo Yuan Zheng Li in the Western Han Dynasty: "What you hear is not as good as what you see, what you see is not as good as what you practice, and what you practice is not as good as what you distinguish by hand." In order to grasp the truth, leading cadres must carry out hands-on investigation and study, and go to the front line to see, listen, ask and think by themselves. This is a working method and an official style.

　　Wen/Zhiqiu

　　Editor/Zhong Qi

　　Dubbing/Jing Xuan

　　Audio Clip/An Xian

　　Poster design/chess

　　Source/People’s Daily, Beijing Daily, etc.