On the morning of March 11th, the Fifth Session of the 13th National People’s Congress held a press conference in the Great Hall of the People, and Li Keqiang, Premier of the State Council of the People’s Republic of China attended the press conference. Xinhua News Agency reporter Yin Bogu photo

　　Reduce taxes and fees

　　Before the end of June this year, the remaining tax credits for small and micro enterprises will be refunded in one lump sum.

　　Reporter from American Consumer News and Business Channel: According to relevant research conducted by the National Finance and Development Laboratory, fee reduction is the biggest help for small enterprises, followed by tax reduction. Can you share with us specific data about the impact of tax reduction and fee reduction? In addition, due to the slowdown in the development of the real estate market, local government finances have decreased. What are your considerations in this regard? And when it comes to consumption, will the government consider issuing consumer vouchers and other similar policy measures?

　　Li Keqiang: The conclusion should be supported by theory and practice. From our practice in recent years, the effect of tax reduction and fee reduction is the most direct. I remember talking to more than a dozen entrepreneurs in the eastern region last year. They talked about the difficulties in the operation of enterprises and hoped that the state would introduce some macro-support policies. I said at that time that the central government’s policy reserves were available, but they needed to be used centrally. There are three choices, but you can only make multiple-choice questions, that is, choose one out of three. First, large-scale investment, maybe you can get an order. Second, issuing coupons may directly stimulate consumption. The third is to reduce taxes and fees for enterprises, stabilize employment and promote investment and consumption. They were silent for a while and almost replied with one voice, we chose the third option. Because this is the most direct, fair and efficient. Judging from the relevant reports I received this year, tax reduction and fee reduction are generally regarded as the first expectation of the government’s macro policy. It seems that fertilization has to be applied to the roots, and the roots can flourish.

　　I also saw from the materials that some people are worried that the marginal effect has been decreasing after the tax reduction and fee reduction has been implemented for several years, that is to say, the effect is not as great as before. The large-scale tax reduction and fee reduction we implemented this time is a combination of tax rebate and tax reduction, with a scale of 2.5 trillion yuan. When the economy was hit hardest in 2020, it was such a large policy scale that we finally survived, and this time we adjusted the structure and put the tax rebate first. The so-called tax refund means that according to the design of the value-added tax system, the market subject is similar to the tax paid first and then returned. We adopt the method of early tax refund, that is, we will refund the remaining tax to the enterprise at one time, with a scale of more than 1.5 trillion yuan. If the effect is good, we will increase our efforts.

　　We give priority to small and micro enterprises in tax refund, because small and micro enterprises are large in quantity and wide in scope, support a large number of employed people, and now it is the most tense and difficult time for them to have funds. Therefore, before the end of June this year, we will refund the remaining tax credits of small and micro enterprises in one lump sum, fully solve the remaining tax credits of some key industries such as manufacturing, R&D services and so on, and solve the incremental tax rebates for small and micro enterprises month by month. When I participated in the discussion in the CPPCC, a CPPCC member was an entrepreneur, and he told me that compared with other measures such as tax reduction and fee reduction and investment, the tax rebate effect came fastest and best. There may be many twists and turns in running a project. Tax refund is equivalent to finding money directly for enterprises and increasing cash flow, which is timely rain. It seems that it is better to say a thousand words than to do something practical, and this key measure must be put in place.

　　Reducing taxes and fees is a subtraction, but it is also an addition in essence, because if you retire today, you will increase tomorrow. If you reduce today, you may increase tomorrow. Last year, the money paid by the new tax market participants exceeded the money we reduced taxes in that year, which is recorded. Since we implemented the value-added tax reform in 2013, it has been reduced by 8.7 trillion yuan under the guidance of tax reduction. At that time, our fiscal revenue was about 11 trillion yuan, and last year it exceeded 20 trillion yuan, nearly doubling. Because enterprises have benefited from this process, the benefits have increased. It can be said that there are many fish in the deep water, which is to conserve tax sources and cultivate and expand market players.

　　Just now, you said that local fiscal revenue has encountered new difficulties, and we have noticed it. Therefore, this year’s increase in transfer payments from the central government to local governments is rare for many years, with an increase of 18% and a total scale of 9.8 trillion yuan. The tax rebate is mainly paid by the central government, and of course, local governments have to "chip in". Our tax refund money goes directly to enterprises. Considering the difficulties of the grassroots, our transfer payment subsidy funds go directly to the grassroots below the city and county. Local governments should be "iron cocks", and they should not spend any money they shouldn’t spend. They should not spend any less money on market players. An extra point would add luster.

　　Situation in Ukraine

　　Support and encourage all efforts that are conducive to the peaceful settlement of the crisis.

　　Reuters: Since Russia launched its attack on Ukraine, two million refugees and hundreds of civilians have died. People are also worried about the prospect of nuclear escalation, but China has never condemned Russia’s behavior or called it "invasion". Will China not condemn Russia anyway? Will China further provide economic and financial support to Russia in the face of sanctions? Are you worried that this will be negatively affected by sanctions from other countries?

　　Li Keqiang: As I said just now, China has always pursued an independent foreign policy of peace. Regarding the situation in Ukraine, China advocates that the sovereignty and territorial integrity of all countries should be respected, the purposes and principles of the UN Charter should be observed, and the legitimate security concerns of all countries should be taken seriously. China makes our own judgment on this basis and is willing to play an active role in returning to peace with the international community.

　　The current situation in Ukraine is indeed worrying. We should do our utmost to support Russia and Ukraine to overcome difficulties and negotiate a peaceful outcome. We support and encourage all efforts that are conducive to a peaceful settlement of the crisis. It is urgent to avoid the tension from escalating or even getting out of control, and the international community and all parties have a consensus on this.

　　China calls for maximum restraint to prevent a large-scale humanitarian crisis. China has put forward an initiative to deal with the humanitarian situation in Ukraine and will continue to provide humanitarian assistance to Ukraine.

　　At present, the world economy is already very difficult because of the impact of the epidemic, and the relevant sanctions will have an impact on the recovery of the world economy, which is not good for all parties. China is willing to make its own constructive efforts to safeguard world peace and stability and promote development and prosperity.

　　stabilize employment

　　Give full play to the wisdom of ordinary people, and the big stage of employment will be colorful

　　Reporter from China Central Radio and Television General Station: At present, we have seen that some enterprises have unstable expectations, some industries have reduced their jobs, and some enterprises have laid off employees. On the other hand, the new population in need of employment is increasing. May I ask the Prime Minister, what measures will we take this year to achieve the goal of stable employment?

　　Li Keqiang: Employment is not only a livelihood issue, but also a development issue. Only when you have employment can you earn money, make a living and create wealth for the society. I remember when I went to the enterprise for investigation last year, a person in charge of the enterprise told me that in August, many employees advanced their wages to him. Why? Children have to pay tuition in September. Before the Spring Festival, I went to the northwest countryside for investigation. A farmer told me that it costs more than 10,000 yuan a year for one of his children to go to college, and more than 8,000 yuan a year for another child to go to high school. It is impossible to grow a few acres of land, but there must be income from working. I am really moved by our people. They are working hard. Working and getting jobs are not only related to the current family livelihood, but also strive for a better future for the next generation.

　　This year, we will strengthen the implementation of macro policies, and fiscal and monetary policies should focus on achieving employment goals. Therefore, we emphasize that employment priority is also a macro policy, and other policies should be matched to achieve employment goals. At present, we must create more than 11 million new urban jobs every year, and it is better to have more than 13 million people. I always feel that as long as we achieve full employment, we can realize what some people call the potential economic growth rate of China. For example, when the epidemic hit hard in 2020, we didn’t set an economic growth target, but we set a clear target, that is, more than 9 million new urban jobs were created, and as a result, more than 11 million new urban jobs were created. The economy not only achieved positive growth, but also the growth rate reached 2.2%, which was the only one among major economies to achieve positive growth.

　　This year, the number of new urban laborers who need employment has reached about 16 million, the highest in many years. There are 10.76 million college graduates, the highest in history. There are also nearly 300 million migrant workers who have the opportunity to work, and they must also ensure the employment of retired soldiers. There are still some enterprises that live and die, and some people want to re-employment. The new labor force in cities and towns is growing, and a new employment platform is needed. For the newly-added people who need employment, we should give them various support measures such as training and solve the employment problem by market-oriented methods. For example, in recent years, we have been promoting mass entrepreneurship and innovation, promoting the development of new technologies, new formats and new models, and cultivating new kinetic energy. We believe that ordinary people are intellectually intelligent, and if their special skills and intelligence are brought into play, the big stage of employment will be colorful.

　　Flexible employment has to be mentioned here, because there are more than 200 million people in this field, with various forms and wide coverage. As a developing country, this form of employment will exist for a long time. It is really hard for them to come and go in the wind and rain, and many places are providing them with warm-hearted services. In view of their labor rights, social security and other issues, the government should gradually improve the policy, that is to say, these "riders" should be tied with "safety belts", so that new employment forms such as flexible employment can not only solve urgent needs, but also stimulate market vitality and social creativity.

　　Optimize the business environment

　　The government must carry out the reform with the cutting edge inward, so that the market players can emerge one after another and be full of vitality

　　Xinhua News Agency: In recent years, although China’s business environment has improved, market players have increased substantially, and enterprises have made things much more convenient, there are still many kinds of interference. What efforts will the government make to optimize the business environment and stimulate market vitality and creativity under the current circumstances?

　　Li Keqiang: In recent 10 years, the State Council has held a national conference every year to promote the "streamline administration, delegate power, strengthen regulation and improve services" reform and optimize the business environment. I also noticed that at the beginning of this year, many places held meetings around the theme of improving the business environment. It can be said that the "streamline administration, delegate power, strengthen regulation and improve services" reform is to improve the growing soil for market players, to reduce taxes and fees is to fertilize and water them, and mass entrepreneurship and innovation are to promote more market players to take root. As long as we bring people’s creativity into play and stimulate the vitality of market players, everyone can imagine the vivid situation of the economy. However, the government must carry out the reform with the cutting edge inward, and can’t let the government departments turn around their own power, but through the reform, the market players will emerge one after another and be full of vitality.

　　It is more difficult to touch the interests than the soul. However, the importance of government is constant. In recent years, we have continued to promote market-oriented reform and the "streamline administration, delegate power, strengthen regulation and improve services" reform. More than 1,000 administrative licenses have been decentralized or cancelled, and non-administrative licenses have withdrawn from the historical stage. In the past, it took dozens of days to get a license for running a business, and it took hundreds of days. Now it takes an average of four days nationwide and one day in the least places. At present, about 90% of government services are online, handheld, off-site, and not met. In fact, this is breaking the barriers of interests and breaking through the traditional thinking. We will continue to promote measures that benefit enterprises and benefit the people.

　　Through the continuous improvement of the business environment in recent years, China’s market entities have reached 150 million, a net increase of about 100 million compared with 10 years ago, mainly private market entities, including 100 million individual industrial and commercial households. Don’t underestimate individual industrial and commercial households, they are connected with the livelihood of many people at one end and the consumption of the public at the other. When I participated in the discussion in the CPPCC, those entrepreneurs said that if small and micro enterprises and individual industrial and commercial households did not get through the "micro blood vessels", large and medium-sized enterprises and even state-owned enterprises and central enterprises would not be able to move.

　　Let’s take a look at the places with good economic development. There are great reforms, good business environment and many market players, so the economy is full of enthusiasm. Of course, I have repeatedly stressed that "liberalization" and "management" go hand in hand. "liberalization" is not a responsibility, but "management" is a duty that the government must perform. "Liberalization" is not laissez-faire. We must resolutely crack down on those acts of counterfeiting, cheating and abduction, especially in some fields involving people’s lives and health and the interests of the masses, such as food and medicine, safe production and finance. Those who violate the law must be punished. Now the new format and new model are constantly changing and developing. We should constantly improve the regulatory regulations and methods so that market players can truly compete and develop in a fair and just environment.

　　Hong kong epidemic situation

　　The SAR government should take the main responsibility of fighting the epidemic, and the central government will fully support Hong Kong in fighting the epidemic.

　　Phoenix TV reporter: Since last year, the Hong Kong Special Administrative Region has held elections for the Election Committee and the seventh Legislative Council. This year, the sixth Chief Executive election will be held. On the one hand, a new electoral system is implemented; on the other hand, the epidemic situation in Hong Kong has not shown signs of improvement, and everyone is very concerned about this election. How does the Prime Minister comment on this?

　　Li Keqiang: At present, the epidemic situation of COVID-19 in Hong Kong is grim. The SAR Government has decided to postpone the election of the sixth Chief Executive according to law and concentrate on fighting the epidemic. We fully understand and support it. The central government pays close attention to the epidemic situation in Hong Kong every day, and is very concerned about the life, health and safety of Hong Kong citizens. The SAR Government should assume the main responsibility of fighting the epidemic, and the central government will fully support Hong Kong in fighting the epidemic.

　　We will comprehensively, accurately and unswervingly implement the policy of "one country, two systems", "Hong Kong people ruling Hong Kong" and a high degree of autonomy, and the change of the SAR government will be carried out in strict accordance with the relevant provisions of the Basic Law. I hope that the SAR Government will unite and lead people from all walks of life in Hong Kong to continue to develop the economy and improve people’s livelihood, consolidate and enhance Hong Kong’s position as the three major international financial, trade and shipping centers, and maintain Hong Kong’s long-term prosperity and stability.

　　epidemic prevention and control

　　Make prevention and control more scientific and accurate according to the change of epidemic situation and virus characteristics.

　　Spanish EFE reporter: It has been two years since the outbreak. In the past two years, China has basically been "closed". Does China now consider making the current "dynamic zero clearing" epidemic prevention and control policy more sustainable? Is there a road map that is "open" to the world?

　　Li Keqiang: Covid-19 is the common enemy of mankind. It has been spreading for two years, and the virus has been mutating. Some laws need to be further studied, and corresponding measures such as vaccine protection and effective drug research and development need to be strengthened. At present, the international community should unite, cooperate and help each other, understand and tolerate each other more, and work hard to create conditions for the world to return to normal.

　　China has been coordinating the prevention and control of epidemic situation and economic and social development, and actively promoting international exchanges and cooperation. We will make prevention and control more scientific and accurate according to the changes of epidemic situation and the characteristics of the virus, so as to ensure people’s life and health, normal production and living order, and the safety of industrial chain and supply chain.

　　Since the outbreak, I have talked with the heads of international organizations, multinational companies and entrepreneurs many times, and they all hope to ensure the necessary business contacts. We have opened "fast track" and "green track" to ensure the normal production and operation of some key enterprises and projects. We will continue to accumulate experience, respond to possible changes in a timely manner, and gradually make logistics and people flow smoothly.

　　Sino-US relations

　　Sino-US cooperation has great potential and is willing to seek long-term benefits with the United States.

　　Bloomberg: Fifty years ago, US President Nixon’s visit to China ushered in the era of American contacts with China. Last year, the Biden administration announced that this era had ended, and the United States and China are now entering a period of fierce competition. Do you agree with this evaluation? Will it become more and more common to ban the sale of American semiconductors in China and the listing of China enterprises in the United States?

　　Li Keqiang: Fifty years ago, China and the United States broke the ice and set sail for the normalization of relations. Half a century has passed, and although the relations between the two countries have been bumpy from time to time, they have been developing forward. We still hope that the two sides will respect each other, coexist peacefully and win-win cooperation in accordance with the consensus reached by the two heads of state at the end of last year, properly control differences in a rational and constructive way, and respect each other’s core interests and major concerns. Still need more dialogue and communication. Since the two sides have opened the door to each other, they should not close it again, let alone "decouple".

　　China and the United States are permanent members of the United Nations Security Council and the largest developing and developed countries in the world. Dealing with each other’s relations is related to the well-being of the two peoples. At present, many global challenges require cooperation and joint response between China and the United States. It should be said that Sino-US cooperation is beneficial to both countries and the world.

　　Of course, there are great differences in social systems, history, culture and development stages between China and the United States, and differences are inevitable. But we believe that cooperation should be the mainstream, because world peace and development depend on cooperation. Even if we have market competition in the economic and trade field, it should be benign and fair competition. Last year, the trade volume between the two countries exceeded $750 billion, an increase of nearly 30% over the previous year. What does this mean? The scope of Sino-US cooperation is vast and has great potential. If the United States relaxes its export restrictions to China, the bilateral trade volume will be even larger, and both countries and their peoples will benefit from it. China is willing to work with the United States to choose a wide range and seek long-term benefits.

　　Support difficult industries

　　Support industries with special difficulties to survive and make people’s lives warm.

　　China News Service reporter: In the past two years, affected by the epidemic, we found that some snack bars and small restaurants that we used to go to have not opened since they closed, including catering, and tourism, retail, passenger transport and other industries have also been greatly affected. The government work report proposes to stabilize market players and ensure employment. May I ask the Prime Minister, what measures will we take this year to help industries with special difficulties?

　　Li Keqiang: After the outbreak, the service industry, especially the contact service industry, was the hardest hit, among which small and medium-sized enterprises were the largest. They have a thin foundation, and they can be said to be careful in calculating and managing day by day, and many difficulties have accumulated to make it difficult for them to support. Helping them is actually supporting employment, because only 100 million individual industrial and commercial households have driven the employment of nearly 300 million people. If the seedlings are dry and the roots are thoroughly rooted, it will be too late to help them. Therefore, we must see that "the weight is too small to weigh a thousand pounds" and we must give them timely support.

　　For these industries with special difficulties, we have introduced more than 40 support policies. Just the tax rebate, roughly speaking, such industries as catering, tourism, passenger transport and culture can enjoy 180 billion yuan. They not only need financial support, but also give "seamless loan renewal" to those with good market prospects. Moreover, support should also be given to those places where they need to reduce rent and electricity charges in stages, which is actually the owner’s pull on customers.

　　At present, consumer demand is indeed relatively weak, mainly due to weak offline consumer demand. As you can imagine, there are many shops in the long lane of the city, and it is steaming, that is, the bustling fireworks. If it is closed, it will not be a good thing, and the lives of ordinary people will be affected. Therefore, we support these industries with special difficulties, not only to make them survive, but also to make people’s lives warm and our economy show more vitality.

　　relations across the Taiwan Straits

　　Compatriots on both sides of the strait are, in the final analysis, a family’s brotherhood, which can be cut off at any time.

　　Taiwan Province Dongsen New Media Reporter: With the epidemic continuing to spread and the uncertainty and instability of cross-strait relations increasing, how will the mainland respond to the situation in the Taiwan Strait and maintain and enhance the well-being of people on both sides of the strait?

　　Li Keqiang: Our major policy toward Taiwan is clear, as I have stated in the government work report, that is, we will adhere to the one-China principle and the "1992 Consensus", resolutely oppose the "Taiwan independence" separatist acts, and promote the peaceful development of cross-strait relations and the reunification of the motherland.

　　Compatriots on both sides of the strait are, after all, a family, and brotherhood can never be cut off. We are willing to continue to share development opportunities with the vast number of Taiwan Province compatriots, provide equal treatment to Taiwan Province compatriots who come to the mainland for development, and do practical things and solve difficult problems for them. As long as compatriots on both sides of the strait work together and unite, they will certainly be able to promote the peaceful development of cross-strait relations and share the well-being of the Chinese nation’s rejuvenation.

　　fulfil one’s duty scrupulously

　　In the last year of this government, we will practice our promises with hard work.

　　Singapore Lianhe Zaobao: This year is the closing year of the current China administration. Looking back over the past four years, what achievements do you think this government has made? What is the biggest challenge? In the last year of this government, China’s economy is facing unprecedented pressure. What policies will you focus on to better stabilize social confidence and respond to people’s concerns?

　　Li Keqiang: Since the current government, the international situation has been complicated and changeable, with domestic contradictions and difficulties superimposed. Speaking of the biggest challenge, it is the COVID-19 epidemic and its severe impact on the economy. We are determined not to seek easy things, not to take refuge in things, and not to avoid risks, and we have done our best.

　　I remember the first press conference I held here when I became Prime Minister, that is, in this hall, I made it clear that it is the basic task of our government to continuously develop the economy, continuously improve people’s livelihood and promote social justice. To be broad-based, people-oriented and benefit the world is to take the expectations of the people as the direction of administration. In recent years, we have been persistent and consistent, innovating the way of governance, coping with cyclical economic fluctuations by keeping the economy running in a reasonable range, especially the implementation of macro policies facing the needs of market players; Firmly promote reform, simplify and eliminate complexity, safeguard justice, cultivate and expand market players and new kinetic energy, and stimulate market vitality and social creativity; Adhere to the priority of employment, grasp the actual situation of the country, pay attention to ensuring basic people’s livelihood, and so on. We are clear that there are some areas where the government work is recognized by the people, and there are also some gaps and shortcomings with their expectations.

　　As for you, this year is the last year of this government and my last year as Prime Minister. The situation we are facing is still complicated and grim, and there are still many difficulties and challenges. I have explained and expounded the difficulties and challenges in the government work report and the questions I just answered. My colleagues and I will do our duty with perseverance and practice our promises with hard work.

　　I believe that under the strong leadership of the CPC Central Committee with the Supreme Leader as the core, with the strong support from all walks of life, especially the joint efforts of the people, China’s economy will certainly be able to climb over the hill, achieve the main objectives and tasks of economic and social development this year, and lay a solid foundation for future development.

　　opening up to the outside world

　　No matter how the international situation changes, we will unswervingly expand opening up.

　　Kyodo News: The China government indicated that it is moving towards the goal of common prosperity, and proposed to set up "traffic lights" to prevent the disorderly expansion of capital, especially to strengthen the supervision of Internet enterprises. However, foreign capital is worried that the China government will further strengthen the restrictions on corporate behavior because of the goal of common prosperity. What is China’s view? Is there any way to reduce investors’ worries?

　　Li Keqiang: When we talk about common prosperity, we should work together. China’s policy of opening to the outside world has not changed and will not change. Moreover, our foreign investment law clearly stipulates that change will only develop in a direction conducive to opening wider to the outside world and investment and trade.

　　Foreign businessmen come to China to develop themselves, but also bring us investment and sales channels and the goods we need, which is beneficial to everyone. Why should we restrict them? Just now I talked about the "streamline administration, delegate power, strengthen regulation and improve services" reform, which emphasizes the need to simplify administration and decentralize power, combine liberalization with management, optimize services, oppose monopoly and unfair competition, and prevent the disorderly expansion of capital. The purpose is to give support to enterprises operating according to law and ensure that all types of ownership enterprises and domestic and foreign-funded enterprises develop equally and compete fairly. Of course, I also hope that enterprises will be standardized and healthy in their development.

　　China is so big that you can find every case you want. But on the whole, China’s economy has been deeply integrated into the world. China’s total import and export of goods has accounted for more than one-third of GDP, and now the comprehensive impact of imports on industry exceeds 70%. We have been the second largest import market in the world for 10 consecutive years, and have become the largest country in global goods trade for 5 consecutive years. Of course, on the whole, we are still in the process of moving from the low-end to the high-end, but it also shows that the industry and consumption are upgrading, the market potential is great, and there is a lot of room for all kinds of investment.

　　Relevant countries and we jointly signed the regional comprehensive economic partnership agreement, namely RCEP, which was officially implemented this year. This can be said to be the largest free trade zone in the world. We will take this opportunity to continue to promote free trade, treat state-owned enterprises, private enterprises and foreign enterprises equally, and continue to make China a hot spot for global foreign investment.

　　I tell you clearly that no matter how the international situation changes, China will unswervingly open wider to the outside world. The Yangtze River and the Yellow River will not flow backwards. Over the past 40 years, China has always been advancing through reform and developing through opening up. As long as it is conducive to expanding high-level opening up, we are willing to do it actively, and we must firmly safeguard the multilateral trading system, which is also the need of our own development.

　　China has been opening to the outside world for more than 40 years, which has developed itself, benefited the people and benefited the world. This is a door of opportunity, and we will never, never close it.

　　(According to Xinhua News Agency, Beijing, March 11 th)

　　On September 8, researchers monitored the operation of the platform in the "Sustainable Development Big Data Platform System". The platform is the first basic platform of big data cloud service for sustainable development in China, which can provide "one-stop" services and data public products for different users such as the public, researchers and decision makers, such as data calculation, analysis, display and sharing services. Xinhua News Agency reporter Jin Liwang photo

　　The 14th Five-Year Plan for the Development of Big Data Industry recently released by the Ministry of Industry and Information Technology (hereinafter referred to as the Plan) proposes that by 2025, the estimated scale of China’s big data industry will exceed 3 trillion yuan, and a modern big data industry system with strong innovation, high added value and independent control will basically take shape.

　　When interpreting the Plan, China Information and Communication Research Institute and China Electronic and Information Industry Development Research Institute pointed out that digital industrialization supports industrial digitalization, and industrial digitalization drives digital industrialization. The Plan systematically lays out the new stage to promote the high-quality development of big data industry. We must seize the opportunity and push the development of big data industry to a new level.

　　Big data industry ushers in a broader development space.

　　Xie Shaofeng, Director of the Information Technology Development Department of the Ministry of Industry and Information Technology, said that the "14th Five-Year Plan" period is a crucial period for China to accelerate the construction of a manufacturing power, a network power and a digital China, which puts forward new and higher requirements for the development of the big data industry, and the big data industry will enter a new stage of integrated innovation, rapid development, in-depth application and structural optimization. He said that data has become the fifth largest factor of production after land, labor, capital and technology. The value of data elements is reflected in two aspects. One is that data is the "oil" in the digital economy era, and the other is that data is the "diamond mine" in the digital economy era.

　　According to the Plan, during the 14th Five-Year Plan period, the development of big data industry should focus on promoting high-quality development, take supply-side structural reform as the main line, take the value of data elements as the guide, focus on consolidating the foundation of industrial development, focus on promoting high-quality data resources, innovative technology and high-efficiency infrastructure, focus on building a stable and efficient industrial chain, focus on improving industrial supply capacity and industry empowerment effect, coordinate development and safety, and cultivate an independent, controllable and open cooperative industrial ecology.

　　According to the Plan, by 2025, the big data industry will maintain rapid growth, the value system will be initially formed, the industrial base will continue to be solid, the industrial chain will be stable and efficient, the industrial ecology will develop healthily, and the modern big data industry system with strong innovation, high added value and independent control will basically take shape. Among them, the estimated scale of the big data industry exceeded 3 trillion yuan, and the compound annual growth rate remained at around 25%.

　　Focusing on releasing the value of data elements, consolidating the foundation of industrial development, building a stable and efficient industrial chain, and building a prosperous and orderly industrial ecology, the Plan puts forward the main tasks of high-quality development of big data industry during the 14 th Five-Year Plan period and deploys key actions.

　　Promote the high-quality development of the big data industry

　　Zhang Li, president of China Electronic and Information Industry Development Research Institute, said that the Plan is based on the realistic foundation of China’s big data industry development, conforms to the law of the times when the industrial economy is striding forward to the digital economy, faces the new direction and new requirements of data factor market cultivation, supports industrial digitalization with digital industrialization, and drives digital industrialization with digital industrialization, which systematically lays out the new stage to promote high-quality industrial development, and is an important guide for the development of China’s big data industry.

　　Zhang Li said that China’s big data industry is welcoming a new round of development opportunities and should vigorously promote the high-quality development of the big data industry.

　　First, strengthen the basic advantage and change the development advantage into the driving force for development. Generally speaking, the advantages of data resources scale, first-Mover advantage in some fields and broad market space are the important support for China’s big data industry to become better, stronger and bigger. We should base ourselves on basic advantages, turn advantages into motivation, fully stimulate the potential of data elements, accelerate the promotion of the big data industry chain to high-end and modernization, continue to deepen the integration of big data and the real economy, and push the development of big data industry to a new level.

　　Second, grasp the core of development and promote the release of the value of data elements. During the "Fourteenth Five-Year Plan" period, to promote the development of big data industry, we must adhere to the concept of data elements, integrate, circulate, apply and manage data elements throughout, accelerate the improvement of data value system and market rules, strengthen the strategic and basic role of data elements in driving economic and social transformation and development, and realize the value release and re-creation of data elements.

　　Third, make clear the main direction of attack and make overall plans to promote the upgrading of industrial base and the modernization of industrial chain. During the "Fourteenth Five-Year Plan" period, in order to promote the high-quality development of China’s big data industry with integrated innovation, in-depth application, structural optimization and ubiquitous empowerment, first, efforts should be made to lay a solid foundation for industrial development, and new infrastructure such as communication, computing power and integration should be deployed appropriately in advance, so as to enhance the ability of technical research and market cultivation, give play to the leading role of standards, and build a solid foundation for industrial development; Second, efforts should be made to improve industrial supply capacity, optimize product supply quality, improve industrial chain, meet the needs of economic and social digital development, and strengthen the supporting role of big data in promoting high-quality economic development and building a new development pattern for services.

　　Fourth, focus on ecological construction and jointly promote the construction of industrial ecological system. During the "Fourteenth Five-Year Plan" period, we should adhere to coordinated development and classified guidance, give full play to the core role of big data enterprises, accelerate the construction of an industrial ecology with resource coordination, subject coordination and regional coordination, and fully stimulate the power and potential of industrial development.

　　Make efforts to make breakthroughs in important links

　　Yu Xiaohui, president of China Information and Communication Research Institute, said that the Plan made a comprehensive deployment around the overall requirements, main tasks and safeguard measures for the development of big data industry during the 14 th Five-Year Plan period. Among them, technological innovation, product services, infrastructure and data security are important links in the development of big data industry. To this end, we should take the initiative to plan and accelerate development, and push the development of China’s big data industry to a new level.

　　First, strengthen technological innovation and seize the autonomy of industrial development. To improve the level of big data technology in China, we should focus on several breakthroughs: comprehensively sort out the current situation of technology development, clarify the advantages and disadvantages of China’s technology, and achieve "solid foundation, promote advantages, fill shortcomings, and strong and weak items"; Reform the establishment and organization of technology research and development projects, strengthen demand orientation, cultivate and develop technology transfer institutions and technology managers in the field of big data, and improve the professional service ability of technology transfer; Accelerate the training and introduction of talents, encourage universities to optimize the specialty settings of big data disciplines, cultivate high-quality technical and skilled talents, and attract big data talents to return to China for employment and entrepreneurship.

　　The second is to optimize products and services and improve competitiveness in key areas. To improve the level of big data products and services in China, we should focus on several breakthroughs: promoting the marketization of resource allocation and further stimulating market vitality; Strengthen financial support and increase investment in research and development of big data basic software and hardware; Innovate big data service models and formats, develop new service models such as intelligent services and operational integration, and cultivate high-quality big data service providers around comprehensive service needs such as diagnosis and consultation, architecture design, system integration and operation and maintenance.

　　The third is to improve infrastructure and strengthen the cornerstone of industry empowerment. To improve the energy level of China’s big data infrastructure, we should focus on several breakthroughs: focusing on data collection and transmission, building 5G and Gigabit optical networks at a high level, promoting the planning and layout of the Internet of Things such as industrial internet, intelligent pipe network and car network, and solidly improving the communication network infrastructure; Comply with the trend of integration, strengthen overall planning and coordination, and optimize the layout of data centers; Sort out the computing power demand at the regional level, coordinate the collaborative construction and scheduling of high-performance computing power and artificial intelligence computing power, and coordinate the computing power infrastructure construction; Grasp the application of data circulation and data industry, build a platform of common ability, and scientifically lay out the infrastructure of data circulation and application.

　　The fourth is to ensure data security and build a firewall for the digital economy. To improve China’s data security protection ability, we should focus on several breakthroughs: strengthening the top-level design of big data security, building a perfect system of laws, regulations, policies and standards to provide protection for data security construction; Strengthen data security technology and product development and application, improve the supply capacity of data security products, and promote the development of data security industry; We will promote the construction of key areas such as data cross-border security management and data security monitoring platform, enhance the ability of risk monitoring and traceability analysis, and strengthen the construction of data security management and control system. (Reporter Zhang Hanqing reports from Beijing)

　　Nepal’s Ministry of Interior: A strong earthquake has killed 3,904 people in Nepal.

　　Nepal’s Interior Ministry said on the 27th that a strong earthquake has killed 3,904 people in Nepal.

　　The number of people killed in Nepal’s strong earthquake rose to 3815.

　　According to the British "Guardian" report, Nepal’s internal affairs officials said on the 27th that at least 3,815 people had been killed in a strong earthquake of magnitude 8 on the 25th. WHO estimates that this strong earthquake affected more than 5 million people.

　　Strong earthquake in Nepal: the international community continues to increase rescue efforts

　　International rescue forces continue to reinforce the disaster area. United Nations spokesman Orla Fagan said that dozens of international rescue teams have rushed to Nepal, and they will fly to the disaster area as soon as possible. At present, there is an urgent shortage of drinking water and food in the disaster area, power supply is interrupted in many places, mobile phone networks are intermittent, hospitals are overcrowded, and roads leading to the epicenter are blocked.

　　253 Chinese employees of Nepal Hydropower Project of Three Gorges Group were trapped in the construction site.

　　The Na Suaka Li Hydropower Station, which was built in Nepal by China Hydropower Foreign Corporation under the Three Gorges Group, suffered serious losses in the earthquake. At present, 253 Chinese employees are still trapped in the construction site. According to the latest statistics of Three Gorges Group, two Chinese employees were killed, one was seriously injured and several were slightly injured in the earthquake. The communication road between the project and the outside world is completely interrupted, and the material reserve is expected to be only enough for 2 days at most.

　　Ministry of Foreign Affairs: Four China citizens were killed in Nepal.

　　China’s Foreign Ministry spokesman Hong Lei said on the 27th that four China citizens were killed in Nepal, including one tourist, one mountaineer on the south side of Mount Everest and two employees of a Chinese-funded company building a project in Nepal. It is understood that before the earthquake, there were about 3,000 to 4,000 China tourists in Nepal. China is sending more flights to try to take back the Nepalese citizens stranded in China as soon as possible.

　　Climbers on the north slope of Mount Qomolangma were evacuated from the safety zone and climbing activities were suspended for ten days.

　　On the 27th, Nima Tsering, deputy director of Xizang Autonomous Region Sports Bureau, told China News Service that at present, Chinese climbers on the north slope of Mount Everest have been evacuated to Shigatse, Tibet, and foreign climbers have also been evacuated to the base camp to rest. At present, all climbers on the north slope have retreated to safety. At the same time, the mountaineering activities on Mount Everest were suspended for ten days for observation.

　　One death, eight injuries and one loss of contact caused by the Everest avalanche in China.

　　On 27th, after contacting Tibet Mountaineering Association, Nepal Mountaineering Association, Nepal Ministry of Culture, Tourism and Civil Aviation, chinese mountaineering association learned that the earthquake in Nepal caused an avalanche on Mount Everest, and one climber in China was killed and eight people were injured, two of them were seriously injured, and one of his friends who participated in the international mountaineering team lost contact.

　　Officials say the number of people killed in Nepal’s strong earthquake has exceeded 3,000 and more than 6,500 people have been injured.

　　The head of the disaster management department of Nepal’s Ministry of Interior said that the 8.1-magnitude earthquake in Nepal has killed 3,218 people and injured more than 6,500 people. At the same time, strong local aftershocks continue to occur, causing inconvenience to disaster relief work and the lives of victims. According to the statistics of the earthquake monitoring department, aftershocks exceeding 4.5 have occurred at least 30 times.

　　Tourism Bureau: There are more than 4,000 tourists in China, and 3,000 are individual tourists.

　　According to the website of the National Tourism Administration, according to the investigation of the travel agency team management system of the National Tourism Administration and the data reported by the provincial tourism commissions, at 12:00 on April 26th, there were about 39 China tourism teams in Nepal, with 1,047 tourists (teams and free travellers). It is estimated that there are about 3,000 individual travelers in Nepal.

　　Nearly 300 thousand people in Tibet were affected by the strong earthquake in Nepal, and the magnitude 7.1 aftershock expanded the disaster.

　　On the 27th, the reporter learned from the Tibetan authorities that the 7.1-magnitude aftershock in Nepal at 15: 09 on the 26th aggravated landslides in some areas of Shigatse, Tibet, and blocked roads in many places. In addition, the climate in the disaster-stricken areas in Tibet has been abnormal recently, with continuous rain and snow, which has greatly affected the earthquake relief work and further expanded the disaster situation.

　　The earthquake killed 20 people in Tibet, and the rescue and disaster relief are facing difficulties.

　　On the 26th, the reporter learned from the official that as of press time, the number of people killed in the earthquake in Nepal in Tibet has increased to 20, and another 4 people are missing, 55 people are injured, 1,191 houses and 1 temple have collapsed, 5,828 houses and 54 temples have been damaged to varying degrees, 58 communication base stations have been damaged, and more than 200,000 people have been affected to varying degrees.

　　The aftershocks are frequent, and the "island" of Zhangmu in Tibet is difficult to pass.

　　At 14: 11 on the 25th, an earthquake of magnitude 8.1 in Boccara, Nepal, spread to Zhangmu Port on the Sino-Nepal border. More than 40 hours have passed, and rescuers are still unable to enter Zhangmu Town. At present, the rescue force is only the last 30 kilometers away from Zhangmu Town. However, the aftershock caused the No.2 bridge in the direction of Nyalam to collapse, and Kangqiao Mingdong was buried by landslide. Not only was the vehicle impassable, but 57 commandos who were hiking to Zhangmu were also forced to withdraw.

　　The earthquake in Nepal has killed more than 2,000 people and strong aftershocks continue to occur.

　　According to official statistics of Nepal, the earthquake has caused at least 2,430 deaths and about 6,000 injuries in the country. The earthquake has killed at least 62 people and injured more than 250 people in India. At least 20 people were killed, 4 people were missing, 58 people were injured and more than 200,000 people were affected to varying degrees in Tibet, China. The earthquake also killed four people and injured more than 200 people in Bangladesh.

　　The number of casualties is rising, and international rescue is racing against time.

　　After the disaster, Nepal declared a state of emergency and appealed for international assistance. On the 26th, a large number of relief supplies, doctors and relief workers from neighboring countries have arrived in Nepal. With the help of the international team, rescuers worked hard to clear the ruins. Due to the limited site conditions, rescuers, including the police and the army, had to dig with their bare hands in an attempt to rescue the survivors under the rubble of the collapsed building.

　　About 350 citizens of China are still stranded at Nepalese airport.

　　Up to now, there are about 350 China tourists stranded in tribhuvan international airport. China Embassy vacated the consular compound, arranged special personnel to receive China citizens who came for help, and more than 150 people were temporarily arranged to spend the night safely in the museum. In addition to Nepalese citizens who are traveling in China, there are more than 2,100 Chinese-funded institutions in China.

　　Click to view the summary of important information:

　　Summary of Important Information of Strong Earthquake in Nepal on 25th

　　Summary of Important Information on 26th Strong Earthquake in Nepal

CCTV News:Buses in Changxing, Zhejiang Province took the elderly out of the countryside, while in Chenzhou, Hunan Province, a passenger bus that entered the village was "transformed" into a courier van because of a large number of online shopping by villagers.

In Suxian District, Chenzhou City, Hunan Province, at 4 pm every day, urban and rural bus drivers have to drive their vehicles from the city to Feitian Village, Suxian District, 28 kilometers away. Different from other trains of the day, there are many online shopping couriers in this car.

Lin Bo, bus driver of Yi Lin Bus Co., Ltd., Chenzhou, Hunan Province:The company uses the 4 o’clock in the afternoon to deliver express delivery. For each express delivery, we have a subsidy of 10 cents and a monthly income of several hundred dollars.

After the "passenger and freight mail" bus entered the village, it ignited the "online shopping craze" of the villagers. From sofas and home appliances to nail clippers, this express delivery point in Feitian Mountain Village has more than 20 express deliveries from local people online every day.

Cao Xijuan, a villager from Feitian Village, Suxian District, Chenzhou City, Hunan Province:I have never used an air fryer before, but now I want to buy it and try it. Nowadays, online shopping is very convenient, and it will take two or three days to arrive. The TV, washing machine and refrigerator at home are all online shopping.

The number of village-level delivery service points like this has expanded to 825 in Chenzhou in the past three years, covering 1342 villages with a coverage rate of 96.96%. In the whole year of 2022, the rural express delivery business in Chenzhou completed 16,959,200 pieces.

Team Xiang Ye of Medical College made a breakthrough in the research of African swine fever virus.

Li Pilong’s research group of School of Life and Li Haitao’s research group of Medical College cooperated to report the new mechanism of histone modification regulating chromatin compartmentalization by promoting phase separation.

The Engineering Laboratory of Medical College reported the key factors regulating the spread of dengue virus and put forward a new anti-dengue blocking strategy.

The research group of Li Yue, Department of Electronics, issued a report on the realization of near-zero exponential concept devices by dielectric integrated optical doping.

Fang Xianyang’s research group of School of Life has made new progress in the solution structure study of long-chain non-coding subgenomic RNA of flavivirus.

Zhu Junming and Xue Lan of the School of Public Administration jointly published a document to reveal the impact of China’s carbon emission trading policy on low-carbon innovation.

Zhong Yi’s research group of School of Life reveals the downstream molecular mechanism of active forgetting of different memory components.

Xiao Bailong from the School of Pharmacy and Li Xueming from the School of Life jointly published a paper in Nature.

Reveal the delicate molecular machines structure and mechanism that mediates human tactile perception.

Team Xiang Ye of Tsinghua Medical College has made a breakthrough in the research of African swine fever virus.

On September 17th, a team led by Xiang Ye, an associate professor hired by Tsinghua University Medical Dean and a researcher at Beijing Advanced Innovation Center for Structural Biology, published an academic paper entitled "Structure of the African Swine fever virus major capsid protein p72" online in Cell Research. In this study, the high-resolution structure of African classical swine fever virus capsid protein p72 was analyzed for the first time by the method of three-dimensional reconstruction of single particle by cryoelectron microscope, and the possible assembly mechanism of African classical swine fever virus capsid was revealed, which laid a solid foundation for the development of African classical swine fever virus subunit vaccine.

African swine fever virus originated in Africa, and the mortality rate of domestic pig infection is nearly 100%, which is a great threat to domestic pig breeding industry and even people’s livelihood and economy. African swine fever virus has spread rapidly from Africa to Europe, South America and some parts of Asia in the past few decades. In August 2018, African swine fever virus infection was found in northeast China, and soon the virus spread to most parts of the country, causing economic losses of more than 100 billion yuan. At present, it is urgent to develop effective vaccines and epidemic prevention measures to control and prevent African swine fever virus. In response to the urgent needs of the country, Tsinghua University Beijing Advanced Innovation Center for Structural Biology and Beijing Frontier Research Center for Biological Structure took the lead, and more than a dozen research groups from Peking University, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Shanghai Pasteur Institute and other units began to tackle key problems of African swine fever virus in April this year.

▲ Structure of African classical swine fever virus: a. Simulate the capsid structure of African classical swine fever virus produced by virus assembly according to the capsid protein p72 structure of African classical swine fever virus; B freeze electron microscopic structure of African classical swine fever virus capsid protein p72 trimer.

African swine fever virus is a double-stranded DNA virus with complex structure. Its genome is protected by two capsids and two outer membranes. The capsids are mainly composed of virus coding protein p72. According to previous experimental data, p72 accounts for about 33% of the total weight of virus particles, and p72 is the main antigen that can be detected in the blood of infected pigs. At the initial stage of studying p72 protein, Xiang Ye’s research group found that p72 protein with correct folding conformation could not be obtained by recombinant expression of p72 protein alone, and only when p72 was co-expressed with another helper protein B602L encoded by African swine fever, could the p72 protein with correct folding conformation be obtained. On this premise, the research team determined the high-resolution 3-D structure of p72 protein of 2.67 angstrom (above) by using the method of single particle 3-D reconstruction with cryoelectron microscope. The results showed that three p72 molecules formed a stable trimer form. Each p72 contains two pancake-like domains connected in series, and each domain consists of eight β-folded sheets, which is very common in the capsid protein structure of icosahedral virus. The six cake-like structures of trimer p72 together form a pseudo-hexamer whose bottom is anchored on the inner membrane of the virus. The inserted fragments between trimer p72 folded sheets together form a propeller-like top structure that extends out of the virus, which is most likely the receptor binding area on the cell surface.

There have been many attempts to study subunit vaccines based on p72 protein of African classical swine fever virus, but no satisfactory results have been obtained. The failure of previous studies is probably due to the failure to use the correctly folded p72 protein. Obtaining the correctly folded p72 protein and determining its high-resolution structure are helpful to develop drugs targeting virus capsid assembly and subunit vaccines. At present, the research and development and evaluation of p72 subunit vaccine are being closely carried out by Tsinghua University and Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences.

Associate Professor Xiang Ye is the correspondent of this article. Liu Qi, a 2014 Ph.D. student from Tsinghua University Life Union Center, is the first author of this paper. Ma Bingting, a 2015 Ph.D. student from Xiangye Laboratory, Associate Professor Tan Xu from Tsinghua University College of Pharmacy and their laboratory members Qian Nianchao and Zhang Fan, and Professor Lei Jianlin, director of the cryo-electron microscope platform of Tsinghua University Life College, participated in this work.

Paper link:

https://www.nature.com/articles/s41422-019-0232-x

Li Pilong’s research group of Tsinghua Life College cooperated with Li Haitao’s research group of Medical College to report the new mechanism of histone modification regulating chromatin compartmentalization by promoting phase separation.

On September 16th, Li Pilong’s research group of Tsinghua University Life College cooperated with Li Haitao’s research group of Tsinghua University Medical College. An academic article entitled "Histone modifications regulate chromatin compartmentalization by contributing to a phase separation mechanism" was published in Molecular Cell.

Eukaryotic cells use many membrane-bound organelles to distinguish biochemical reactions in order to better regulate space and time. For example, their genomes are enclosed in a membrane-bound organelle, namely the nucleus, in order to better control the genetic information flow. In the nucleus, the genome is further regulated to form many compartments or regions, which play an important role in regulating gene expression. Judging from some of their characteristics, these dynamic solid compartments are similar to membraneless organelles. Many protein that play a role in chromatin compartmentalization have the inherent ability of liquid-liquid separation, such as human heterochromatin protein HP1α and Drosophila heterochromatin protein HP1a. Based on these observations, it has been suggested that chromatin compartmentalization is formed by liquid-liquid separation. However, the previous studies can’t explain why the other two homologous proteins, HP1β and HP1γ, of human heterochromatin protein HP1α, which can’t be separated by themselves or from DNA, perform similar functions, and whether the formation of constitutive heterochromatin is regulated by the liquid-liquid separation mechanism formed by multivalent interaction between multivalent HP1 protein complex and multivalent histone H3K9 trimethyl (H3K9me3).

▲ Liquid-liquid separation mediated by multivalent interaction between H3K9me3 on nucleosome string and CD of HP1 complex promotes the formation of heterochromatin compartments.

In this work, Li Pilong and Li Haitao’s research group cooperated to prove that in vitro, the modification of histone H3K9 trimethyl (H3K9me3) and the multivalent interaction of recognizing its modified HP1 chromatin domain Chromodomain(CD) can lead to the formation of heterochromatin. The authors used two polyvalent HP1-CD complexes, the key chromatin fragment (NE) extracted from human nucleus and the nucleosome string (NA) modified by H3K9me3 in vitro as experimental systems. Several evidences show that the formation of these droplets is mediated by liquid-liquid separation. The resulting droplets are reminiscent of the dynamic behavior of heterochromatin and its response to biochemical regulation and mutant phenotype. The author concludes that the liquid-liquid separation driven by multivalent H3K9me3/CD interaction is the key force for the formation of heterochromatin in cells. To sum up, this study proposed a general mechanism of histone labeling regulating chromosome compartmentalization by promoting liquid-liquid separation.

Li Pilong and Li Haitao are co-authors of this article. Wang Liang, a 2015 Ph.D. student from Tsinghua University Life College, Gao Yifei, a 2016 Ph.D. student from Medical College, and Zheng Xiangdong, a postdoctoral fellow from Medical College, are the co-first authors of this article.

Paper link:

https://www.cell.com/molecular-cell/fulltext/S1097-2765(19)30658-6

Cheng Gong Laboratory of Tsinghua Medical College reported the key factors regulating the spread of dengue virus and proposed a new anti-dengue blocking strategy.

On September 16th, Professor Cheng Gong from Tsinghua University Medical College published an academic paper entitled "Host serum iron modulations of dengue virus acquisition by mosquitoes" (doi: 10.1038/S41564-019-0555-) in Nature Microbiology. This study found for the first time that the iron content in human serum is the key factor to regulate the transmission of dengue virus by mosquitoes, and put forward an anti-dengue transmission blocking strategy based on iron supplementation.

Dengue fever is one of the most widespread viral infectious diseases in the world. Dengue virus is carried by mosquitoes and spread to people. At present, dengue fever has been prevalent in more than 100 countries and regions around the world. According to the estimation of the World Health Organization, about 2.5 billion people in the world live in areas threatened by dengue infection, 390 million people are infected or repeatedly infected with dengue virus every year, and 500-1 million people are hospitalized. Dengue fever has become the largest insect-borne viral infectious disease in the world. In recent 20 years, the epidemic trend of dengue fever in the world has intensified, which has become a worldwide public health problem. Aedes aegypti and Aedes albopictus are the primary vectors of dengue virus transmission in nature. This kind of mosquitoes are widely distributed in tropical and subtropical regions, but the epidemic trend of dengue fever is not completely consistent with the distribution of mosquitoes, and there are obvious population and regional differences in dengue fever. Identifying the key factors affecting the spread of dengue virus is a key scientific problem to be answered in this field.

Mosquitoes can acquire viruses from infected hosts by sucking blood. Within a few days after mosquitoes suck blood, the infected person’s blood is quickly digested. At the same time, the virus in the blood infects the intestinal epithelial cells of mosquitoes and spreads into the body cavity of mosquitoes, so that mosquitoes have the ability to spread viruses. The above two processes are highly coincident in time, so researchers speculate that host blood factors or blood metabolites can regulate the process of virus infection in mosquitoes, and ultimately determine the vector efficiency of mosquitoes carrying and spreading viruses. Through a series of screening, it was found for the first time that the iron ion content in the host serum was highly negatively correlated with the ability of mosquitoes to acquire virus by blood-sucking, and it was proved that the iron ion concentration in the serum was the key negative regulatory factor to inhibit mosquitoes from acquiring virus infection. The results of further research show that iron ions in serum can be directly utilized by the iron metabolism system of intestinal cells after entering the intestinal tract of mosquitoes, which inhibits the expression of antioxidant enzyme genes in cells, leading to a significant increase in the level of reactive oxygen species (ROS) in the intestinal tract of mosquitoes, and finally inhibits the infection and amplification of viruses in mosquitoes (Figure 1).

▲ Figure 1. Host serum iron ions can increase the level of reactive oxygen species in the intestinal tract of mosquitoes and inhibit the infection of dengue virus in mosquitoes by inhibiting the expression of antioxidant enzyme genes in intestinal cells.

Iron deficiency is a nutritional problem widely existing in nature. According to the World Health Organization, iron deficiency is widely distributed in Africa, South America, Southeast Asia and other regions. By comparison, it can be found that the distribution area of iron deficiency is highly coincident with the epidemic area of dengue fever, so the researchers speculate that the iron deficiency state of the population is closely related to the epidemic of dengue virus. In the subsequent study, it was found that the low iron state of the host can promote mosquitoes to infect the virus during blood feeding, and iron supplementation to the low iron host can block the spread of the virus. It shows that universal iron supplementation is an effective means to cut off the spread of dengue virus in nature, which will provide a new prevention and control idea and strategy for dengue virus prevention and control.

▲ Figure 2. Iron supplementation to the host can block mosquitoes from carrying and spreading dengue virus.

Professor Cheng Gong of Tsinghua University Medical College is the correspondent of this paper, and Zhu Yibin, a postdoctoral fellow of Tsinghua University Medical College, is the first author. Professor Wang Penghua from the Medical College of the University of Connecticut, Professor Liu Qiyong from the Institute of Infectious Diseases of the Chinese Center for Disease Control and Prevention, Professor Bian Zhongqi from the Department of Infectious Diseases of the 920th Hospital of China People’s Liberation Army Joint Logistics Support Force, Professor Itsanun Wiwatanaratanabutr from Thailand Xianhuang Institute of Technology, and Wu Tianshi, deputy chief physician of Tsinghua University School Hospital, are the collaborators of this paper.

Paper link:

https://www.nature.com/articles/s41564-019-0555-x

The research group of Li Yue, Department of Electronics, Tsinghua, published a report in Nature Communication on the realization of near-zero exponential concept devices through dielectric integrated optical doping.

On September 11th, the research group of Associate Professor Li Yue of Tsinghua University Department of Electronic Engineering published a research paper entitled "Substance-integrated photonic doping for near-zero-index devices" in Nature Communications. It is the first time to propose an optical doping method that can be integrated with dielectric to realize functional devices with near-zero exponential electromagnetic characteristics, which provides a new design method for new chip integrated circuits and has potential application value in biosensor, integrated circuits, functional materials and other fields.

▲ Conceptual diagram of dielectric integrated optical doping: (a) linear structure, (b) bending structure.

Near-zero-index device is a kind of functional device based on near-zero index medium. Compared with traditional electromagnetic devices, it has the characteristics of deformation, local adjustment and field enhancement. Electromagnetic wave in near-zero exponential medium presents a special wave effect of spatial static distribution, including near-zero refractive index and group velocity, as well as infinite wavelength and phase velocity. In 2017, Professor Nader Engheta and Associate Professor Li Yue of the Department of Electrical and Systems Engineering of the University of Pennsylvania jointly published an article in the journal Science, proposing the concept of optical doping based on near-zero exponential media for the first time, transplanting doping technology from micro-scale to macro-scale, and regulating the magnetic permeability of equivalent media through doping in non-ordered media, thus realizing multi-modal control such as paramagnetic, diamagnetic and ideal magnet. It is a kind of aperiodic structure.

▲ Experimental observation of optical doping characteristics of dielectric integration: (a) test platform, (b) near-zero reflection, zero phase and high group delay.

Based on the engineering application of the new theory, this paper puts forward an optical doping method based on dielectric integration. Combining with typical integrated circuit technology, the regulation of optical doping on the magnetic characteristics of equivalent media is expressed as the regulation of the electromagnetic response of chip integrated devices, and a variety of near-zero exponential functional devices are realized. For example, a high-sensitivity biosensor with the help of local field enhancement effect in doped media, an efficient photoacoustic modulator with weak mechanical vibration, and an "electric fiber" (analogous to optical fiber) that can be bent and deformed at will and its working frequency remains unchanged. In addition, Li Yue team verified the electromagnetic characteristics of the concept of dielectric integrated optical doping through experiments, and observed zero phase shift, high time delay, position independence of doped media and device shape independence, which were consistent with the analytical theoretical prediction and full-wave simulation results.

The theoretical and experimental work of this thesis was completed in Tsinghua University, and the Department of Electronic Engineering of Tsinghua University was the first unit of this thesis. Li Yue and Nader Enkhtar are the corresponding authors of this article, and Zhou Ziheng, a doctoral student in the Department of Electronic Engineering, is the first author. Other authors include Li Hao and Sun Wangyu, Ph.D. students from Tsinghua University Department of Electronic Engineering, and Dr. Inigo Liberal from Navarre Public University.

Paper link:

https://www.nature.com/articles/s41467-019-12083-y

Fang Xianyang’s research group of Tsinghua Institute of Life has made new progress in the study of solution structure of long-chain non-coding subgenomic RNA of flavivirus.

On September 10th, Fang Xianyang’s research group of the School of Life cooperated with Qin Chengfeng’s research group of the Institute of Microbiology and Epidemiology of the Academy of Military Medicine. A research paper entitled "Long non-coding subgenomic RNA of flavivirus has extended 3D structures and is flexible in solution" was published in EMBO Reports. In this paper, the solution structure of long-chain non-coding subgenomic RNA from flavivirus type 2 dengue virus, Zika virus and West Nile virus was reported in detail for the first time.

▲ Flow chart of studying the structure of flavivirus sfRNA solution by SAXS and computational simulation.

Long non-coding RNA (lncRNA) is a non-coding RNA with a length of more than 200 nucleotides, which plays a variety of important functions in organisms and is a hot spot in life science research. Like its host, almost all viruses can encode long-chain noncoding RNA. Many mosquito-borne flavivirus, such as dengue virus, Zika virus and West Nile virus, are pathogens that pose a great threat to human health. A large number of long-chain non-coding subgenomic RNA(sfRNA) will accumulate in the infected host cells, which play an important role in the pathogenicity of the virus and the immune escape from the host.

It is of great significance to study the structure-function of long-chain noncoding RNA, but at present, there is no effective means to study its three-dimensional spatial structure, so the three-dimensional spatial structure information of long-chain noncoding RNA is still very little. The length of long-chain non-coding subgenomic RNA from mosquito-borne flavivirus is between 400 and 500 nucleotides, so it is very difficult to study its structure by using traditional structural biology methods, such as X-ray crystallography, nuclear magnetic resonance or cryoelectron microscopy. Fang Xianyang’s research team and their collaborators, combined with small-angle X-ray scattering technology and computational simulation, studied the solution structure and flexibility of long-chain non-coding subgenomic RNA from dengue virus type 2, Zika virus and West Nile virus by step-by-step solution. It is found that the long non-coding subgenomic RNA of flavivirus has multiple subdomains, which have an extended three-dimensional spatial structure in solution, modular structural characteristics similar to multi-domain proteins and great flexibility.

It is particularly interesting that the three-dimensional spatial shapes and structures of the dumbbell-shaped domains in series of sfRNA of Dengue virus, Zika virus and West Nile virus based on small-angle X-ray scattering data are significantly different, which is presumed to be caused by the topological differences of their pseudo-knots in series. This inference is confirmed by further computational simulation, in vitro biochemistry and in vivo cell experiments. This study is an active exploration of the three-dimensional structure of long-chain noncoding RNA. The research results of this paper not only provide important structural information for understanding the physiological function of sfRNA, but also show that the integrated structural biology method based on small-angle X-ray scattering and computational simulation is a feasible method for studying the three-dimensional structure of other long-chain noncoding RNA solutions.

Fang Xianyang, researcher of Tsinghua University Life College, and Qin Chengfeng, researcher of Institute of Microbial Epidemiology, Institute of Military Medicine, Academy of Military Sciences, are co-authors of this article. Zhang Yupeng, a 2013 direct doctoral student of Tsinghua University Life College, Zhang Yikan, a 2016 general doctoral student, Dr. Liu Zhongyu, an associate professor of Institute of Microbial Epidemiology, and Cheng Mengli, a master student of Institute of Microbial Epidemiology, are co-authors of this article.

Paper link:

https://doi.org/10.15252/embr.201847016

Zhu Junming and Xue Lan of Tsinghua University of Public Administration jointly published a document to reveal the impact of China’s carbon emission trading policy on low-carbon innovation.

On September 9th, Associate Professor Zhu Junming and Professor Xue Lan from School of Public Administration of Tsinghua University published a research paper entitled "Low-carbon innovation induced by emissions trading in China" online in the international academic journal Nature Communications, and evaluated the effect of the pilot policy of carbon emissions trading in China on promoting enterprise innovation and its mechanism.

Carbon emission trading is a widely concerned market-based carbon emission reduction policy, which has been adopted by many countries and regions in the world and can play an important role in mitigating climate change. At present, the empirical knowledge about the effect of carbon emissions trading policy mainly comes from the study of EU carbon market. However, people still have little understanding of the scope of this market-oriented policy, the influence of interaction with other policies and the changes in the mechanism brought about by policy design, which limits the further promotion and greater role of the policy.

In order to solve this problem, Associate Professor Zhu Junming and Professor Xue Lan from School of Public Administration of Tsinghua University pay close attention to the pilot policy of carbon emission trading in China since 2013, and use enterprise-level data and patent application information to empirically test the influence and potential mechanism of the policy on low-carbon innovation of enterprises by using the methods of matching and double difference. Low-carbon innovation is helpful to get rid of the path dependence on carbon-intensive economy and energy infrastructure, and alleviate the negative impact caused by the uncertain trend of carbon emission reduction, which is very important to achieve stable emission reduction.

▲ Patent application trends of carbon trading enterprises and non-carbon trading enterprises.

It is found that, on the basis of the impact of policies such as energy conservation and emission reduction and low-carbon pilot projects, carbon emission trading has promoted trading enterprises to increase 1.75 low-carbon patent applications within two years, which is close to the policy effect of two low-carbon patent applications in the EU carbon market within five years, and at the same time, it has increased the applications of other types of patents, especially other green technology patents. The influence scope of the policy goes beyond the carbon emission trading enterprises, and the influence on enterprises that have not been included in carbon trading but may be included in the future is also very significant. It is also found that the innovation behavior of enterprises is not affected by the price of carbon trading, but by the way of quota allocation. This series of research findings provide a reference for the construction of China’s national carbon market.

Associate Professor Zhu Junming from the School of Public Administration of Tsinghua University is the first author and co-author of the article, while Deng Xinghua, a former postdoctoral fellow in Tsinghua University and now a lecturer at Southwestern University of Finance and Economics, is the co-author of the article, and Professor Xue Lan and Fan Yichun, an undergraduate graduate of Tsinghua University and now a master of Massachusetts Institute of Technology, are the co-authors of the paper.

Paper link:

https://www.nature.com/articles/s41467-019-12213-6

Zhong Yi’s research group of Tsinghua Institute of Life reveals the downstream molecular mechanism of active forgetting of different memory components.

On September 5th, the research group of Zhong Yi, School of Life Sciences, Tsinghua University published a research paper entitled "Genetic Dissection of Active Forgetting in Labile and Consolidated Memories in Drosophila" in PNAS.

In this paper, the downstream molecular mechanisms of active forgetting of unstable short memory and stable long memory in Drosophila were systematically compared and analyzed for the first time. It was found that the active forgetting of unstable short memory was mediated by Rac1/WAVE complex/ Dia pathway, while the active forgetting of stable long memory was mediated by Cdc42/WASp/ Arp2/3 complex pathway. As Dia and Arp2/3 complex are reported to regulate the linear growth and branching growth of neurofilament skeleton respectively, the research results of this paper provide an interesting possibility: the active forgetting of memory components with different stability may be related to the growth mode of different forms of neurofilament structure.

▲ Analysis of forgetting mechanism of stable memory and unstable memory.

As early as 1885, Hermann Ebbinghaus, a German psychologist, described the forgetting curve of the human brain for newly acquired memories, and began the journey of human beings to explore the mystery of forgetting. In the following 100 years, many scholars tried to find the mechanism of forgetting by using the method of experimental psychology, and also created a variety of theories to explain the phenomenon of forgetting, but ultimately failed to get a unified answer. As a basic feature of human memory, forgetfulness has been widely studied and reported in psychology, but almost no one cares about it in the research of neurobiology, and it has become a "forgotten corner". The main reason may be that the forgetting process is simply regarded as another description of memory failure, and it is a passive process (if you remember poorly, you will forget quickly, and if you remember well, you will forget slowly), thus ignoring the unique molecular mechanism of forgetting itself.

Until 2010, Professor Zhong Yi’s research group discovered the biological mechanism of active forgetting for the first time, that is, the learning process itself will activate the molecular pathway of forgetting signals to actively forget the formed memories. This active forgetting mechanism does not affect the quality of learning itself, but specifically affects the speed of forgetting acquired memories. Moreover, different memory components will have their own active forgetting mechanisms. For example, in the olfactory punishment learning of Drosophila, one training (5 minutes) can produce two completely different memory components at the same time: unstable anesthesia-sensitive memory (ASM) and relatively stable anesthesia-resistant memory (ARM) which lasts for more than one day. Zhong Yi’s research group has found that a study session can activate Rac1 molecule in the small G protein family to erase the unstable anesthetic sensitive memory that lasts for several hours, and activate Cdc42 molecule, another member of the small G protein family, to forget the more stable anti-anesthetic memory that lasts for more than one day. However, the specific downstream molecular pathways of the forgetting mechanism mediated by Rac1 and Cdc42 are not clear.

Recently, the research paper published online by Zhong Yi’s research group analyzed the downstream molecular pathways of these two forgetting mechanisms for the first time: Rac1/WAVE complex/ Dia pathway mediates the active forgetting of short-term memory components (unstable anesthesia-sensitive memories that last for several hours), while Cdc42/WASp/ Arp2/3 complex pathway mediates the active forgetting of long-term memory components (relatively stable anti-anesthesia memories that last for more than one day). Both of these active forgetting mechanisms occur in the mushroom body of the learning and memory center of Drosophila (the mushroom body contains about 4000 neurons, and the whole brain of Drosophila has more than 100 thousand neurons). Interestingly, cell biology research found that Dia was directly responsible for the linear growth of microfilament skeleton, while Arp2/3 complex was responsible for the branching growth of microfilament skeleton. This suggests that the active forgetting function of different memory components may be related to different forms of dynamic changes in the microfilament skeleton structure of neurons. On the one hand, these research results bring the academic understanding of the molecular mechanism of forgetting into a more detailed dimension, on the other hand, they provide more effective pharmacological targets for directly regulating the function of forgetting.

Gao Yang, a Ph.D. student in the School of Life Sciences of Tsinghua University, Shuai Yichun, a researcher in the Janelia Research Campus of the United States, Zhang Xuchen, a graduate doctoral student in the School of Life Sciences of Tsinghua University, and Peng Yuwei, a doctoral student, are the co-first authors of this paper. Professor Zhong Yi from Tsinghua University and Assistant Researcher Li Gan are co-authors of this article.

Paper link:

https://doi.org/10.1073/pnas.1903763116

Xiao Bailong of Tsinghua Pharmaceutical College and Li Xueming’s research group of School of Life jointly published a paper in Nature.

Reveal the delicate molecular machines structure and mechanism that mediates human tactile perception.

On August 21, Nature (Nature) The journal published the research paper "Structure and Mechanizing of the Mammalian Tactical Channel Piezo2" written by Xiao Bailong’s research group of Tsinghua University Pharmaceutical College and Li Xueming’s research group of College of Life Sciences. The three-dimensional structure and exquisite working mechanism of high-resolution cryoelectron microscope, a mechanical force molecular receptor-PIEZO 2 ion channel, which gives human tactile perception ability, are reported for the first time. This research is another important research achievement in this field after the two research groups reported in Nature on January 22nd, 2018 the high-resolution three-dimensional structure and molecular mechanism of another member of the mechanically gated PIZO channel family-PIZO 1 ion channel, which not only strongly promoted the understanding of the structural basis and molecular mechanism of PIZO channel family, but also laid a solid foundation for drug development based on PIZO channel. At the same time, Nature published a commentator’s article and spoke highly of the research.

Touch, as one of the five senses, not only gives us the ability to perceive pleasant touches such as shaking hands, stroking and kissing to maintain normal social behavior, but also bears the biological basis for human beings to skillfully use various tools such as touch screen, mobile phone or mouse. However, under pathological conditions such as tissue injury or inflammation, abnormal tactile perception function can lead to severe mechanical hypersensitivity (also known as touch pain). For example, patients with cancer or arthritis often suffer from severe pain caused by slight touch such as dressing or walking, which seriously affects individual health and quality of life. In addition, autistic people usually show more sensitive tactile perception than ordinary people. Recent studies suggest that this abnormal tactile function may be one of the important causes of autism.

Tactile perception originates from the response of mechanical force sensing molecular receptor-mechanically gated cation channel expressed on primary sensory neurons to mechanical force stimulation, which causes extracellular cations, such as sodium ions and calcium ions, to flow into cells, which in turn induces nerve cell excitement and signal transmission, and finally leads to tactile sensation. In 2010, the research group of Professor Ardem Patapoutian of Scripps Institute in the United States identified that the Piezo gene family encodes the necessary components of mechanically gated cation channels in mammals. In 2012, Dr. Xiao Bailong, who is engaged in postdoctoral research in Professor Patapoutian’s research group, and his colleagues reported in the journal Nature that Piezo protein constitutes the core pore component of mechanically gated cation channels, thus establishing a brand-new ion channel family type of mechanically gated Piezo channels for the first time.

Subsequent studies have proved that Piezo2 mediates the mechanical perception of tactile, proprioception (such as posture balance perception) and visceral perception (such as lung contraction and expansion, blood pressure perception and heart rate regulation) in mammals, while Piezo1 has been found to act as a molecular receptor of mechanical force in a variety of cell tissues and participate in regulating the development of blood vessels and lymphatic vessels, blood pressure homeostasis, bone formation and remodeling and many other functions. The genetic mutation of Piezo gene has been found to cause many human genetic diseases, including erythrocytopenia syndrome, lymphatic edema, distal joint contracture, tactile loss and so on. The human body with the loss-of-function mutation of Piezo2 not only shows tactile and proprioception defects, but also loses the perception of mechanical hypersensitivity in pathological state, which confirms that Piezo2 channel can be used as an important target for developing new analgesic drugs.

▲ Figure A, schematic diagram of frozen electron microscope of Piezo2, in which each subunit is represented by a different color; B, a schematic diagram of a dome-shaped structure surrounded by three outward twisted transmembrane paddle areas, and the transmembrane area is marked by the green dotted line; C, schematic diagram of the topological structure of Piezo2 with 38 transmembrane regions; D, a subunit structure of Piezo2 constitutes a display diagram; E, Piezo2 central pore module area; F, the central pore area of Piezo2 and Piezo1 surrounded by IH-CTD; The radius distribution diagram of the central pore area of G, Piezo2 and Piezo1, including the switch gate of transmembrane area and the Constriction neck)； of intracellular area; H, Piezo1 and Piezo2 mutants with deletion of hat region lack the current induced by mechanical stimulation.

Dr. Xiao Bailong’s research group comprehensively uses the multi-disciplinary research methods, such as biochemical structure, electrophysiological patch clamp, Qualcomm dosage drug screening, transgenic mouse model and human genetics, and focuses on solving the key scientific problems of how the mechanically gated Piezo channel converts mechanical stimulation into electrochemical signals, and how it uses its own mechanical sensitivity and channel characteristics to determine related physiological and pathological functions, and is committed to developing new drugs and technologies targeting Piezo channel. Up to now, as a correspondent (including co-author), he has made a series of important research achievements in the three-dimensional structure analysis, molecular mechanism revelation, small molecule drug discovery and physiological and pathological function exploration of Piezo channel.

In this latest Nature paper, relying on the technical system and research platform established by two research groups for analyzing the structure of Piezo1 channel by cryo-electron microscope, the researchers have made unremitting efforts for more than six years to overcome the difficulty of extremely low expression of mouse-derived Piezo2 with a total length of 2,822 amino acids. Through continuous exploration and optimization of protein purification conditions and sample preparation methods by cryo-electron microscope, a stable and homogeneous protein sample was finally obtained for data collection by cryo-electron microscope. In the process of structural analysis, Piezo protein is extremely flexible, which brings great challenges to the resolution of high-resolution structures. The researchers expanded the symmetry of Piezo2 data particles, cut them into three parts, and calculated them independently, so as to obtain high-resolution structures of each part and then put them together into a complete structure, overcoming the flexibility problem, and finally obtained a three-dimensional structure with an overall resolution of 3.6-3.8 angstroms (Figure A), and successfully analyzed the complete topological structure of Piezo2 protein including 38 transmembrane helical regions (Figures C and D).

The researchers found that the overall three-dimensional structure of Piezo2 channel is similar to that of Piezo1 channel previously analyzed, and it is assembled into a trilobal propeller-like structure with 114 transmembrane helical regions in the form of homotrimer (Figure a-d), and it is established that Piezo channel is a kind of large membrane protein with the most transmembrane times so far. Among them, the 1st to 36th transmembrane helix regions (TM1-36) are sequentially assembled into 9 repetitive structural units with the 4th transmembrane helix region as the unit, and this characteristic structural unit was previously named THU (Transmembrane Helical Unit) with the abbreviation of Tsinghua University (Figure c, d). Very interestingly, these nine thus are connected in series to form a special non-planar transmembrane "Blade" structure, which leads to the three blades enclosing into a dome-shaped structure with a diameter of 28nm and a depth of 10nm (Figure B). Researchers speculate that this dome-shaped PIZO protein-cell membrane system may be one of the structural foundations of PIZO channel with high mechanical sensitivity.

The extracellular "Cap" domain located above the central pore region is completely embedded in the bottom of the dome (Figures A and B), while there is a 9nm long Beam structure inside the cell, which connects the peripheral end of the blade to the intracellular position of the central pore region (Figures a-e). The 38th transmembrane helix (named inner helix, IH) and the trailing intracellular carboxyl terminal domain (CTD) form a central pore part (Figures E and F), including a narrow neck located in the transmembrane region and cytoplasmic region (Figures F and G), suggesting that the Piezo channel structure is in a closed state. The structural comparison between Piezo2 and Piezo1 shows that the transmembrane region narrow site is completely closed in Piezo2 structure, but open in Piezo1 (Figure f, g). Based on this, the researcher proposed that the narrow site forms a switch gate of transmembrane region (Figure f, g). Further structural analysis and electrophysiological function experiments show that the gate may be controlled by the rotation of the top hat domain (Figure H). However, because the narrow constriction neck located in the cytoplasm region is in the closed state in both Piezo1 and Piezo2 structures (Figure f, g), it remains to be further proved whether this site is controlled by the conformational changes of the blade and long rod structure as another switch gate.

Dr. Xiao Bailong said that through the full cooperation with Dr. Li Xueming’s research group, the analysis of the structure from Piezo1 to Piezo2 not only strongly promoted the understanding of the structure and mechanism of mechanical gating of Piezo channel in mammals, but also provided a solid foundation for exploring the mechanism of human diseases caused by the dysfunction of Piezo channel and the identification and discovery of related drugs. Relying on the all-round research platform and academic accumulation of Piezo channel established by their research group, they will devote themselves to the drug discovery and development of Piezo channel.

Dr. Xiao Bailong from Tsinghua University College of Pharmacy and Dr. Li Xueming from College of Life Sciences are co-authors of this paper. Wang Li, a postdoctoral fellow at Tsinghua University College of Pharmacy, Zhou Heng, a 2015 doctoral student at School of Life, Zhang Mingmin, a 2013 doctoral student, and Liu Wenhao, a 2016 doctoral student, are the first authors. In addition, Dr. Deng Tuan, Zhao Qiancheng (now a postdoctoral fellow at Yale University) and Li Yiran of Xiao Bailong’s research group also participated in some research work. Dr lei Jianlin of Tsinghua University cryo-electron microscope platform provided help for data collection of cryo-electron microscope.

Paper link:

https://www.nature.com/articles/s41586-019-1505-8.pdf

Graphic | School of Medicine, School of Life, Department of Electronics, School of Public Administration, School of Pharmacy

Editor | Li Jing

Qingyuan released April sunny and rainy at first sight.

It coincides with the warm spring and flowers.

It’s a good time to step into spring.

Accompanied by frequent turnover of personnel

It is easy to cause diseases such as respiratory tract and intestine.

Qingyuan CDC reminds citizens

When going out to play

Attention should be paid to safety and health first

Be careful in April.

The following seven diseases

hand-foot-and-mouth disease

Hand-foot-mouth disease is mainly caused by enterovirus EV71, Coxsackie virus A16, Coxsackie virus A6 and other enteroviruses. The incubation period is 2-10 days (average 3-5 days). The clinical manifestations are mainly fever and rash or herpes on hands, feet, mouth and buttocks. It spreads through respiratory droplets and faeces, and it is easy to occur in infants under 5 years old. It can occur all year round, and it is spring from April to July.

Guidelines for disease prevention

1. Vaccination with EV71 vaccine can significantly reduce the risk of serious illness and death. In order to play a protective role as early as possible, children are encouraged to complete the whole vaccination before the age of 12 months.

2. Nursery institutions strictly implement the regulations on the management of infectious diseases, implement morning and afternoon inspections, check the causes of absence due to illness, and report the clustered epidemic situation in time.

3. Maintain good hand hygiene, wash your hands before and after meals and after going out and returning.

4. Closed public places and rooms should be ventilated frequently to keep the air circulating. Frequent contact parts, daily necessities and children’s toys are cleaned and disinfected regularly.

whooping cough

Pertussis is a highly contagious acute respiratory infectious disease caused by Bordetella pertussis infection. Since the wide implementation of DTP vaccine immunization, the incidence of this disease has been significantly reduced. However, in recent years, the number of reported cases of pertussis in our city has been on the rise, and some cases have typical paroxysmal and spasmodic cough, with a course of 2-3 months.

Guidelines for disease prevention

1. Vaccination is one of the important methods to prevent pertussis. Children in China should be vaccinated with one dose at the age of 3, 4, 5 months and 18~24 months, and school-age children should be vaccinated with pertussis in time.

2. Try to avoid exposure. If there is a definite infected person at home, the infected person should follow the doctor’s advice and take antibiotics for treatment in time, and pay attention to ventilation at home to reduce close contact with other family members. In particular, infants who have not been vaccinated at home should try to avoid contact with infected people or live in the same room.

3. Strengthening ventilation, washing hands frequently, wearing masks and other preventive measures can effectively prevent diseases transmitted through respiratory tract.

Tick-borne diseases

Acari, generally reddish brown or taupe, long oval, flat back and abdomen, is only the size of a grain of rice before blood sucking, and can expand several times to dozens of times after blood sucking. Ticks mostly live in grassland, farmland, bushes or wooded environment and animal body surface. As the weather warms up, ticks gradually enter an active period. Ticks can spread many diseases after biting people, such as Lyme disease, forest encephalitis, tick-borne hemorrhagic fever, Q fever, relapsing fever, spotted fever, rabbit fever, tick-borne typhus, human granulocytic anaplasmosis, Bartonella infection and so on.

Guidelines for disease prevention

1. Try to avoid sitting and lying for a long time in the main habitats of ticks, such as grasslands and Woods. Do personal protection and wear long-sleeved clothes; Fasten the trouser legs or stuff them into socks and shoes; Don’t wear sandals; Don’t dry clothes on the grass or trees.

2. After you come back from forests, grasslands and other places, you should pay attention to checking clothes and various parts of your body to ensure that there are no ticks attached; If you bring a pet, you should also carefully check the whole body of the pet to prevent ticks from being carried home in the pet hair.

3. After being bitten by ticks, it is generally not recommended to handle it by yourself. It is best to go to the hospital for treatment.

dengue

Dengue fever is an acute mosquito-borne infectious disease caused by dengue virus, which is mainly transmitted by the bite of Aedes albopictus (commonly known as "flower mosquito"). Mainly popular in tropical and subtropical Southeast Asia, Western Pacific, America and Africa. Dengue fever has the characteristics of typical import and suddenness, rapid spread, high incidence, general susceptibility of the population and high mortality of a few severe cases.

With the increase of temperature and rainfall, the density of mosquito vectors increases, which does not rule out the possibility of local infection caused by imported cases in some areas.

Guidelines for disease prevention

1. Wear long-sleeved clothes when going out and try to choose light-colored clothes; Mosquito repellent products such as mosquito repellent can be used in exposed parts of the skin; Install screen doors, screens and other physical protection measures; Avoid staying in the shade, shrubs, grass and other mosquitoes densely.

2. Reduce the breeding of Aedes mosquitoes

Sealing: sealing the water tank, sealing the sewer manhole or installing anti-mosquito devices and sealing useful utensils.

Fill: Fill potholes, waste ponds, ditches, bamboo holes and tree holes.

Dredging: dredge ditches, clean up silt and weeds on the shore.

Row: clear the accumulated water.

Cleaning: remove small containers of garbage, as well as garbage containers such as plastic film bags, discarded bottles and cans.

Novel coronavirus infection

At present, the epidemic situation of novel coronavirus in our city shows a low level of epidemic fluctuation. novel coronavirus has not disappeared, but it is still mutating, and there is still the risk of infection. The general public still needs to do a good job of self-protection.

Guidelines for disease prevention

1. Take the initiative to vaccinate COVID-19 vaccine, wash hands frequently, ventilate more, gather less, maintain good hygiene habits, and wear masks scientifically in crowded places.

2. Enhancing self-immunity and improving self-immunity is an important means to prevent various diseases. Please ask the general public to maintain a healthy lifestyle such as regular work and rest, exercise, drink plenty of water, eat healthily and have a good attitude.

flu

Influenza is an acute respiratory infectious disease caused by influenza virus, which has the characteristics of short incubation period, strong infectivity, rapid spread, etc., mainly manifested as acute onset, severe headache, chills, fever, body aches, fatigue and other symptoms, which are easy to cause outbreaks; High incidence in winter and spring, the whole population is susceptible, pregnant women, infants, the elderly and patients with chronic basic diseases are high-risk groups, and serious complications may occur.

Guidelines for disease prevention

1. Vaccinate against influenza. Vaccinate against influenza is the most effective means to prevent influenza, which can significantly reduce the risk of serious complications of vaccinators. The virus is easy to mutate, so it is recommended to vaccinate once a year, especially for the elderly, children, pregnant women, patients with chronic diseases, caregivers and medical staff.

2. Maintain good personal hygiene, wash your hands frequently and correctly, avoid dirty hands touching your eyes, nose and mouth, cover your nose and mouth when coughing or sneezing, and wash your hands in time.

3. Reduce going to crowded places and wear masks scientifically.

4. Ventilation indoors to keep the air circulating.

5. Have a balanced diet, moderate exercise and adequate rest to avoid excessive fatigue.

chickenpox

Chickenpox is an acute respiratory infectious disease caused by varicella-zoster virus, which occurs frequently in winter and spring. It is characterized by patches, papules, herpes and scabs on the skin and mucosa in batches with mild systemic symptoms. The incubation period is long, generally 14-16 days (ranging from 10-21 days) after exposure to the virus. From 1-2 days before the outbreak of chickenpox infection to the scab of the rash, the infected person is contagious. It is mainly transmitted through respiratory tract and direct contact. April-June and November-January are the high incidence seasons of chickenpox every year.

Children aged 1 -12 are susceptible, and chickenpox is highly contagious. About 90% of susceptible people will get sick after contacting patients, commonly known as "face-to-face transmission". Places where people gather, such as kindergartens and schools, are prone to outbreaks.

Guidelines for disease prevention

1. Vaccination with chickenpox vaccine is the most economical and effective measure. Chickenpox vaccine is suitable for people who are susceptible to chickenpox at the age of 1 and above. Vaccination is recommended for school-age children and people with high exposure.

2. Nursery institutions, schools, etc. strictly implement the regulations on the management of infectious diseases, implement morning and afternoon check-ups, check the causes of absence due to illness, etc. Once suspicious patients are found, they should be disposed of in time, and standardized disinfection should be carried out on the items they have contacted.

3. Maintain good personal hygiene habits. Wash your hands frequently, eat a balanced diet, strengthen physical exercise, and try to avoid contact with patients with acute chickenpox.

4. Strengthen environmental ventilation. Closed public places and rooms should be ventilated frequently to keep indoor air circulation and clean environment.

5. Patients must be treated in isolation at an early stage until all herpes scabs or not less than two weeks after illness.

Qingyuan fafa editorial department

Original title: "Qingyuan CDC Reminder: Seven Infectious Diseases Need to be Focused on in April"

Read the original text

Recently, a news in the field has aroused widespread concern. It is reported that some owners of Xiaomi models found a new function called "lap time assessment in ranking mode" when receiving the update of Xiaomi HyperOS system. This function claims that if the owner can achieve the officially recommended lap time on the designated track, he can unlock the ranking mode.

This news quickly fermented on the Internet, which triggered heated discussions and doubts among car owners. However, the situation quickly reversed, and many car owners confirmed that Xiaomi had cancelled this OTA update.

In the face of this storm, Xiaomi officially responded quickly. They said that the company has always been committed to establishing good relations with users and attaches great importance to users’ feedback and interests. In the future, before OTA push, we will strengthen communication and forecast with users to ensure the transparency of information and users’ right to know.

According to a car owner of Xiaomi SU7 Ultra, Xiaomi’s sales staff has contacted him actively, apologizing for the incident and offering a car wash machine and points as compensation. The owner said that although the track mode is not used frequently for him personally, he still appreciates Xiaomi’s positive response and compensation measures.

"I think Xiaomi’s handling of this incident is very sincere," the owner said. "They plan to launch a new version of the car machine within 4-6 weeks, and provide 5000 points and car washing machines as compensation for the owners who are locked in power. You can also enjoy the service of free pick-up and delivery of cars to upgrade new cars. "

This incident not only tested Xiaomi’s crisis handling ability in dealing with emergencies, but also highlighted the importance of user feedback to enterprises again. Xiaomi’s quick response and positive compensation measures have undoubtedly alleviated the dissatisfaction of car owners to some extent.

However, different car owners have different views on this incident. Some car owners think that Xiaomi’s compensation measures are sincere enough, while others hope that Xiaomi can pay more attention to the actual needs and experiences of users in future product updates.

　　CCTV News:The plot is always so similar. When people temporarily forget the barbaric growth, a sensational social news brings the rental platform under the chain home to the public’s attention.

　　On October 16th, a netizen named "My free neighbor is a fugitive" posted in Weibo that on October 13th, there was an incident of chopping people with a knife in a free shared rental house. Afterwards, there was blood all over the floor and walls of the rented house, and the smell of blood lingered, making other tenants unable to live, but they freely failed to give other tenants a reasonable explanation.

　　The authenticity and specific details of the incident have not yet been determined, but from the "deadly formaldehyde room" to the "horror camera", it claims to have the freedom of "artisan spirit" and has fallen into a number of negative public opinion whirlpools in recent months.

　　The tenant hacked people in the room. Manager: "What has affected you?"

　　On October 16, 2018, Weibo netizen "My free neighbor is a fugitive" posted that a fugitive with a criminal record had been living in his free room in Haidian, Beijing for half a year, and he hacked someone with a knife in his shared room last Saturday. After the incident, the room was covered with blood, but freely did not give other users a reasonable explanation.

　　Party Weibo (screenshot)

　　At the same time, there was a video posted on the Internet, in which there was a pool of blood on the floor of the room, bloody footprints spread all over the room, and white chairs and walls were splashed with stars. ……

　　In a recording widely circulated on the Internet, the manager’s complaint about the tenant’s anger seems quite indifferent: "I’ll have someone buy you some incense and make some fragrance in the house." In order to show his "sincerity" in handling this matter, the manager also asked the tenant what flavor of air freshener he liked.

　　In the subsequent dispute, the manager confidently asked the tenant, "What happened to you (in the house)?"

　　The authenticity of the recording can not be tested for the time being, and there is no public denial in terms of freedom.

　　After the public opinion fermented, the free customer service responded in Weibo that the matter was an emotional dispute between Ms. Zhang, a free tenant, and two men. The injured person was her ex-boyfriend and the injured person was her current boyfriend. Ms. Zhang is a free tenant, and neither the injured person nor the victim is a free tenant. He also said: the poster made a rumor that Ms. Zhang, the tenant, was a fugitive, which seriously infringed on the reputation of the company and Ms. Zhang, and freely reserved the right to pursue the legal responsibility of the rumor maker.

　　The reporter of "Legal Evening News" contacted the tenant. According to him, after the incident, due to the heavy smell of blood in the room, the poster contacted the free staff, hoping to change the similar room in the same area freely, but was rejected. If you want to "dry the blood", the tenant can go back to live that night. After the tenant refused, he freely said that he could stay in a hotel below 300 yuan for one day, and the company would reimburse him, and then he would live in the rented house after the matter was settled. "　

　　According to the owner, the tenant was taken to see the house after the incident, and only the poster failed to change the rent successfully, or the price was slightly different from the incident room because of the size and location of the room.　

　　At present, the incident continues to ferment, and there is no updated explanation from the free side, and no other actions have been seen in public relations.

　　"Privacy and life are the same."

　　Free official website (screenshot)

　　On the official website, a rental platform owned by Chain Home, there are a row of rooms to be rented: Mo Landi-colored walls and bedding, simple and neat iron or wooden furniture, and the sun shines through bright windows. This is a tenant’s ideal good residence, which meets the various needs of young people for a warm, decent, safe and orderly life.

　　At the beginning of its birth in 2011, it was free to provide an ideal home for young people struggling in big cities, and chain home executives also advertised the "ingenuity" of the company many times in interviews.

　　However, the word "ingenuity" was particularly ironic after a self-media article titled "Alibaba P7 employee died of leukemia and rented a free formaldehyde room before his death" was screened in September this year.

　　Ali, a 37-year-old employee, died of leukemia in less than two months after staying in a free room with excessive formaldehyde. Since then, the Beijing News reporter visited a number of freely rented houses and found that some houses suspected that formaldehyde exceeded the standard. According to the national standard, the indoor formaldehyde standard is 0.1 mg/m3, but the reporter found that the data was as high as 1.018 mg/m3 in a living room, which exceeded the standard by 10 times. Excessive formaldehyde has caused many tenants to have headaches, dry and itchy throats.

　　This is not the first time that formaldehyde in the room has exceeded the standard. However, in the first-tier cities where land is scarce and it is not easy to live in a big place, mature and high-quality housing is quite scarce, and the urgent need for a place to live makes people reduce their consideration of living safety to the second place. It’s just that it’s hard for a rental platform that prides itself on "artisan spirit" to make use of consumers’ eagerness to fish in troubled waters, lower the requirements for housing quality and earn "ambiguous money".

　　Some netizens said that they are a "green radish" who lives in a free room and sucks formaldehyde for free. Behind this black humor is the helplessness and protest of tenants.

　　In the face of surging public opinion, I freely apologized first, then promised that the newly collected houses would be vacant for 30 days, and then rented out after passing the formaldehyde test. In addition, I also took remedial measures such as returning the rent and measuring formaldehyde for free, which was an account for the tenants.

　　For an enterprise that has exposed multiple problems in risk control, management and compliance, "calm" has become a luxury.

　　The "pinhole camera incident" will be freely pushed to the forefront of public opinion.

　　The camera found by the party Chen Cheng (pseudonym) at home (Source: Network)

　　The client Chen Cheng (pseudonym) said that on the evening of September 11th, he found a "strange little hole" on the bedside socket, and it was suspected that a pinhole camera was installed. After reporting the case, it was confirmed by the police that there was a sneak shot device hidden behind the socket.

　　The tenant said that things have been reflected for more than a month, and the company has never given a solution. During this period, only one director stated that this matter has nothing to do with the company.

　　On October 14, the incident was exposed from the media and quickly fermented. On the 15 th, I finally made a public response freely: I have cooperated with the public security organs to file a case for investigation. It is natural to emphasize that the company has strict process inspection and system control on the decoration and configuration of newly-received and second-rented houses. After the incident, the safety management center of the company will further sort out and investigate the hidden dangers with the quality management, decoration and configuration and lease management departments.

　　On the morning of 16th, I had an interview with the tenant freely. However, at the end of the interview, the tenant said that he was free to propose another room change for himself, but he would not change rooms until the dust settled.

　　One after another, the lawsuits that have not yet "settled the dust" consume the reputation that you have been proud of for many years. Some netizens commented with intense emotions: "Living freely, privacy and life are the same." Search Weibo, and you can even see users with the nickname "Free to go out of business today".

　　Does an apology work or not?

　　In 2018, Didi apologized to the public twice for the "flight attendant murder case" and "Wenzhou girl murder case", each time being more sincere. In the open letter, Cheng Wei and Liu Qing reflected on their mistakes to the depths of their souls, saying that "the competitive heart overshadowed the initial heart" and "the lack of awe". In the eyes of the public, this did not save Didi’s reputation, more like "crocodile tears."

　　There are self-media predictions, and a free apology should not be far away.

　　As a traditional format upgraded by the Internet, it stands out in the rental market where "black intermediaries" are everywhere, focusing on better housing, stricter management, more transparent procedures and more humanized services. It is precisely because of this that young people are willing to pay hundreds or even thousands of dollars to choose it every month.

　　In recent years, snowballs have been getting bigger and bigger. In June of this year, it freely announced its achievements, saying that it has settled in nine cities across the country and managed 600,000 houses, claiming to be "the largest professional leasing institution in China".

　　However, there are frequent problems in management. In addition to several vicious incidents sorted out in the article, in the winter of 2017, in the special action of large-scale investigation, large-scale clean-up and large-scale rectification of potential safety hazards in Beijing, the partition room that was free from compliance was demolished, resulting in many households having nowhere to go; Social news about disputes over housekeeping, cleaning and household finance is also frequently seen in newspapers.

　　Looking at the free negative news, unlike Didi, it is not the business logic that has been criticized, but the procrastination and casual attitude after the incident, which is contrary to the entrepreneurial spirit it has always advocated. From indifference to formaldehyde housekeepers, to inaction in "camera incident" and "hacking incident" … … Free "arrogance" keeps consumers’ "prejudice" escalating.

　　So, here’s the problem. Where does the "arrogance" come from?

　　Internet companies provide services with matching information as the core to meet people’s needs of "food, clothing, housing and transportation". At the same time, there is also a huge "information gap" between consumers and enterprises. If you want to buy the convenience and comfort brought by this information, you must endure the "store bullying" of Internet companies.

　　Therefore, there are complaints on the Internet. In real life, on the flashing screen in the hands of "commuters", it is still dripping to match suitable vehicles for them; Young people who have just graduated still have to go back to the rented "free room" to settle down for a day’s fatigue.

　　This is a dilemma. As netizens said, "Didi is better than a black car" and "Free is better than ‘ Black intermediary ’ Good. "

　　In this dilemma, freely, Didi began to "pretend to sleep". They seem to forget that behind the massive information they have obtained are ordinary users; The market on which the pursuit of profits depends is also made up of ordinary users. They seem to forget that technology and algorithms can certainly create a more convenient life, but if consumers are regarded as dirt, it will only be a matter of time before word of mouth collapses and reputation goes bankrupt.

　　Marx once said: "Capital comes into the world from head to toe, and every pore is dripping with blood and dirty things". To clean the pores for the capitalists, it is far from enough to rely solely on their consciousness and conscience. In the past six months, Internet companies have intensified their rectification efforts, Didi has been interviewed and freely interviewed, and the "teeth" of supervision have become sharper and sharper.

　　Life is more than a room. The beautiful declaration of freedom is still hanging in official website, but they have no further explanation or public relations behavior in view of the fog of the "chopping people" incident.

　　Will the future be good?

　　"I don’t know in the future, 2013 is better. At that time, their services were not bad. " In an interview with a reporter, someone said so.

　　That year, Tencent invested 15 million yuan to take a taxi, and the iphone version of the APP was officially launched. That year, we came into contact with a brand-new concept — — "The Internet creates a better life for people".

　　Recently, a master’s thesis entitled "Sociological Research on Fart" was circulated on the Internet, causing controversy. Some netizens are "sarcastic": Cold Window has studied hard for so many years, and it is really a "fart". Some netizens commented after reading that things that seem meaningless and are sneered at are "serious things to dig into."

　　The Paper noted that the paper was written in 2007 by Gao Jianwei, an in-service master of sociology at Huazhong Normal University. At that time, Gao Jianwei not only passed the master’s thesis defense with this paper, but also was rated as "excellent".

　　As early as 2015, the paper had aroused public concern. Li Yaxiong, a tutor of Gao Jianwei and a professor at Huazhong Normal University, said in an interview with the media at that time, "There are actually many aspects in life that are worth exploring, and there is no need to always repeat some meaningless topics. Some topics that look different or niche are often more worthy of consideration and there will be more interesting discoveries."

　　In this regard, Zheng Zuoyu, a professor at the School of Sociology of Huazhong University of Science and Technology, believes that whether the paper can be written well depends on whether the attitude is correct. In his view, even if the topic is high, but the attitude is not good, "no use"; On the contrary, if the academic attitude is correct, the topic "No matter how bizarre it is" can also be described as a classic.

　　The thesis was praised by the tutor.

　　"I am often mentioned by people around me such a question: How did you do such a topic?" Gao Jianwei explained at the beginning of his thesis "Sociological Research on Fart" that he chose this topic because he accidentally saw two stories about fart on the Internet. In both stories, the protagonist changed his life track because of a fart, which triggered Gao Jianwei’s thinking.

　　Gao Jianwei believes that "fart" is not only a physiological phenomenon, but also a social phenomenon, which has various correspondences with social relations. Through the study of a large number of historical materials, he pointed out that fart exists as a social taboo in both western culture and eastern culture. This taboo not only spreads in all aspects of our daily life, but also widely exists in the superstructure fields such as education, manners and customs, habits, morality and even law.

　　Gao Jianwei deeply analyzed how fart behavior and phenomenon changed from a physiological behavior to a social taboo from the perspective of social genetics, and revealed the complex mechanism of this operation process. In the process of social development, fart has evolved from indiscriminate human behavior to behavior that occurs more frequently among some social members, and then to negative behavior in moral evaluation, and finally evolved into a form of social taboo.

　　The paper mentions that in the early stage of human beings, division of labor and class have not yet appeared, and fart behavior is primitive human beings — — That is, the most frequent and common physiological phenomenon of our ancestors. With the development of productive forces, social relations have changed, and the diet types and habits of the rich have changed, and they no longer fart frequently. At this point, the human fart experience has completed the first historic change, that is, from a common physiological phenomenon of human beings to a behavior that occurs more frequently and more commonly among some members of society (more poor people).

　　Through this transformation, fart behavior has gained some social significance, reflecting the fact that the upper and lower classes coexist and oppose each other. In order to maintain its dominant position, the upper class not only establishes an absolute control position in the political, economic and cultural fields, but also strives to "shape" the social state that adapts to its dominant position. Distinguish the upper and lower classes by etiquette, self-cultivation and taste. The upper class is often considered polite, while the members of the lower class are ordinary, vulgar and even despicable. In this context, farting is labeled as a negative moral label. Under the power of discipline, "fart" has changed from a negative social behavior defined by the upper class to a social taboo, and has been recognized and obeyed by all members of society.

　　He believes that this genetic process comes from the strange characteristics of power operation, namely, duality, dependence and publicity. Then it discusses the fart behavior and phenomenon of "no taboo", and thinks that this phenomenon essentially reflects the taboo existing form of fart behavior and phenomenon, and is related to the strange characteristics of power operation.

　　The Paper noted that this is not the first time that this paper has been concerned by netizens. In 2015, Beijing Evening News reported on this matter. Li Yaxiong, Gao Jianwei’s tutor and professor of Huazhong Normal University, was quite appreciative of the paper when interviewed.

　　According to his memory, when the paper was opened, the teachers all laughed at the first sight of the topic, but after the "laugh", everyone quickly discussed its academic value from a professional point of view, not simply talking about the matter, but using sociological theory for analysis. Li Yaxiong told the Beijing Evening News reporter at that time that Gao Jianwei’s research found a new way. In the case of repeated research, it was "precious" and teachers generally supported it.

　　"The defense effect is also very good, and it has also been rated as excellent. He (Gao Jianwei) has a good writing style and clear thinking. " Li Yaxiong said that because it is not convenient to do actual investigation, Gao Jianwei presented the genesis of fart, its vertical development in different times and the social meaning behind it through literature analysis and quotations. "I welcome this topic that comes from life and has humanistic care."

　　In this regard, The Paper recently contacted Professor Li Yaxiong several times for an interview, but the other party declined on the grounds of being away on business.

　　College teachers: With a correct attitude, no matter how strange the topic is, it can become a classic.

　　In fact, in recent years, many papers have attracted public attention because of their unique topics. Previously, papers such as "The Utopian Cat: The Identity and Fantasy of Cats Fans" and "The Influence of Illicium verum on the Volatile Flavor of Braised Chicken and Its Mechanism" have caused heated discussions.

　　"This is a question of diversification of topics, which I think is a good thing." Rui Liu, tutor of master students in Huazhong University of Science and Technology, believes that the development of disciplines is characterized by "specialization" and "cross-integration", and "many problems that we didn’t think could be studied before have emerged now". "Personally, I think it is necessary to study such different problems, which can cover many blind spots of previous knowledge and inspire the society." Rui Liu said in an interview with The Paper recently.

　　In this regard, Zhou Tingting, an associate professor at the School of Journalism, Huazhong University of Science and Technology, believes that college students are active in thinking or familiar with new media, so they can really pay attention to some new phenomena. "Many phenomena can be used as the research topics of our thesis, but we should find an in-depth observation angle based on the discipline, not just describe ‘ Phenomenon ’ It is meaningless to do a simple analysis. " Zhou Tingting said.

　　Tan Songhua, a member of the National Education Advisory Committee, said that he had not read the above papers and could not comment on them. "How to write a graduation thesis, it is difficult to have a unified statement. Students with different majors, hobbies and energies have different factors to consider when choosing a topic. " Tan Songhua said that as far as he guides graduate students, the practical significance is more important when choosing the topic of the thesis, for example, it can promote the development, reform and social progress of the country.

　　For more and more "unique topics", Zheng Zuoyu, a professor at the School of Sociology of Huazhong University of Science and Technology, said that "there is nothing wrong with it". "Academic research should have cared about your life and the society you live in. Now that the era in which students live is full of these phenomena, it is natural to study these phenomena. " According to Zheng Zuoyu, the question is not whether the research topic is "innovative" or "serious", but whether the research attitude is correct.

　　Zheng Zuoyu said that individual students "lead a muddled life" and "choose the most mindless topic" when writing their papers. "Such a topic, even if it sounds tall, is actually vague." Zheng Zuoyu said that attitude is more important for writing papers.

　　"When I take part in the insurance research interview, I will ask students what fields they are interested in and have studied." Zheng Zuoyu found that most students from rural areas would say "I want to do rural research", or most female students would say "I want to do gender research". "But not many of these students have really studied the relevant knowledge. They just think that because of their identity, they can make some sense at worst. " Zheng Zuoyu believes that in this case, even if the topic is tall, the attitude is not correct, and it is "no use"; On the contrary, if the academic attitude is correct, the topic "No matter how bizarre it is" can also be described as a classic.

　　What do students think about the topic of the thesis? Li Yong (pseudonym), a graduate student in a university in Hunan, told The Paper that according to his observation, the source of the topic mainly has three parts, one is the combination of previous practical activities, the other is the combination of interests, and the third is from the tutor. "I prefer topics that I am interested in, and at the same time consider the significance of research, so I am motivated to write." Li Yong believes that interest is a good entry point. "When the school informs you to write a paper, you often feel confused and then choose a familiar field." (The Paper reporter He Liquan intern Hu Tianyi)

　 　Special feature of 1905 film network　The summer file in July, also known as domestic protection month, saw eight domestic films released on July 8 alone. Among them, there is a literary film "Winter" with little publicity before, which will "go to war" on the same day as "Chill 2", "Rock Tibetan Mastiff", "Big Fish Begonia" and "never gone", and the box office prospect is really not optimistic.

The film released on July 8 　 　

　 　Looking at the literary films in the summer file, Winter is not fighting alone. The Roadside Picnic, which will be released on July 15th, will also face fierce challenges from other major rivals, such as The Great Reversal starring Xiao Wa, day trader the Quick Shooter starring Zhang Jingchu and Kenny Lin CP, and the article director Virgo Lu Yao Zhi Ma Li.

The film released on July 15th. 　 　

　 　 It seems to be a right thing for literary films to hide in the domestic protection month, but it can only be said that the risk is small in the schedule. How does the literary film break through the schedule dilemma and get higher film arrangement and box office? Is it difficult for every literary film to kneel like Fang Li? In the final analysis, this should be combined with the promotion of literary films themselves and the development status of mainland art theaters.

　 　 In the Mainland, there was a real representative of the cinema, who explained the discovery of literary films and the development dilemma of art cinemas. She was the program and promotion manager of Beijing Broadway Film Center — — Yang Yang.

　 　　Program and Promotion Manager of Beijing Broadway Film Center — Yang Yang

　 　 The survival status of art cinemas is inseparable from the prosperity of the literary film market, so Yang Yang’s viewpoint is also representative, which is difficult to copy, because there is only one art cinema in the mainland. "The Broadway Film Center has done statistics. The ratio of literary films to commercial films is 1: 10, but the attendance rate is only 1:3. It can be said that all the money is earned from commercial films." From these words, it is not difficult to see that it is almost impossible for a cinema to maintain its operation only by literary films, even if it is an art cinema.

Beijing Broadway Film Center

　 　 Then the question came, and people began to think whether literary films should be shown in art theaters for a long time. But from Yang Yang’s words, we can see that after the establishment of the Art Cinema, there seems to be no improvement in the "way out for literary films". What’s the problem? Is it that the publicity of literary films is not in place, or is it unreasonable to arrange films in cinemas? Why do we see so few literary films when there are art cinemas in the Mainland?

　 　 With these questions, Zi Shijun specially went to Beijing Broadway Film Center and had a good chat with Yang Yang about the announcement of domestic literary films and the operation of art cinemas.

[See the manager of the art theater talking about the myth of domestic literary films]

　 　 Broadway Film Center, established in December 2009, is a Hong Kong-owned art cinema, located in a residential area of Xiangheyuan Road in Dongzhimen, operated and managed by Hong Kong Anle Film Co., Ltd. Before the opening of this one in the Mainland, Anle had successfully operated an art cinema in Hong Kong called Hong Kong Broadway Film Center. With the experience of successful operation, the founding concept of Broadway Film Center in the Mainland also wants to be consistent with it, to provide screening for high-quality literary films at home and abroad, and to expand the exchange of film culture.

　 　 Yang Yang’s job is to select excellent films from the domestic literary films every year, and implement the corresponding film arrangement and post-screening exchange activities. During this period, she also saw the problems faced by mainland literary films, large and small. She told us that "several small partners in her team can even be regarded as a small company for the publication of mainland literary films for a long time".

Earlier, we were equivalent to a literary film publishing company.

　 　 In the early years, some domestic literary films with relatively small volume did not try to be released nationwide at all, such as Cao Baoping’s Glorious Wrath. Before its release, Beijing Broadway Film Center was almost equivalent to their publicity company, which did everything from posters to brochures, as well as writing all press releases and inviting media.

The implementation of Yang Yang’s various ideas was completed on this desk.

　 　 Q: Does this mean that for these small-scale literary films in the early days, do you still announce them here?

　 　 A: In the early days, this movie was very small. I think there was a big difference in the past two years from "Fireworks by Day", but "Fireworks by Day" is a special case that can’t be copied. Since then, plus the rapid growth of the whole China movie market, everyone wants to go to the national release, hoping to replicate a miracle in it.

You cann’t just lay out the whole line at every turn.

　 　 Q: You said earlier that the team was unprofessional, such as selling dog meat by hanging sheep’s head?

　 　 A: Isn’t that what commercial movies are all about? Just find a selling point, whatever it is, just drag it there first.

　 　 Q: What do you think are the unprofessional behaviors of domestic literary films in propaganda?

　 　 A: The announcement of an art film is a difficult thing to do all over the world. Its idea is not for profit, and it is not made for the maximum benefit. So when you announce it, you have to be patient and take a long line. Is there anything better that can bring out its commercial benefits? Like now, there is no particularly good model. I have also asked Anle’s colleagues in Hong Kong, who also bought some small non-Hollywood films in Hong Kong very early, but I feel that they don’t have a particularly good model, including Taiwan Province, but they are all very small. If you follow that, the distribution of these films in China is not particularly bad.

　 　 Q: Do you think that these domestic ideas of putting on a full-screen screening are actually wrong?

　 　 A: I think this idea may need to be changed, because before, only the film center was on the long line, and this volume was a little too small. I understand that they are not satisfied with only showing it in one theater, and I also think it needs to be expanded, but it is unrealistic for you to really compare it with commercial films, even with Fireworks in the Day.

　 　 Q: Before "Fireworks in the Day", there was a saying, why not learn from the United States, first show it on a small scale, and then expand it? Do you think it is difficult to realize this?

　 　 A: To a certain extent, the current publicity companies can’t settle down to do this. If it’s small, you have to choose a cinema and talk about it one by one. Now there are so many publicity weeks for a film, and there are so many cycles for a film. After this project is over, he will immediately enter the next project, no matter what happens to the film in the later stage, but these films must be fermented by word of mouth for a long time before they can be done slowly.

Many literary films will be wasted if they do this.　

　 　 But generally speaking, it is hard to achieve any box office miracle, that is, you accumulate slowly, but now no one is doing this, and there are not even a number of cinemas, even if only 20 or 30 people want to do this, and now it is Broadway, with at most an intermediate cinema. I don’t know what other cinema companies are like, but there are a few cities in Broadway that are suitable for our own company, and then the managers are willing to cooperate. They also know what to do, but they still can’t become a mass.

　 　 In fact, many domestic literary films may not be publicized, and many cinemas simply don’t arrange them. Zi Shijun interviewed some cinema managers when "A Hundred Birds at the Phoenix" was released. Their first reaction was, I haven’t seen it, I don’t know about it.

There is a horse on the other side of the mountain, with a domestic box office of 240,000.

　 　 For example, there is a horse on the other side of the mountain released in May, and the number of films is even lower. The Broadway Film Center has never seen the film, because Yang Yang is familiar with Huang Lu, and she took the initiative to find the producer through her, and then told them whether to come and have a post-screening exchange. "But they haven’t done some movie-watching activities before, and the director happened to be going to other places, and then Huang Lu couldn’t come because he had to shoot, feeling that they gave up on propaganda, and many films would be wasted."

Next page:Is the art cinema really the savior of literary films?